{"product_id":"biolegend-112705","title":"Biolegend, 112705, PE anti-mouse CD210 (IL-10 R) Antibody, 50μg","description":"\u003cp\u003eCD210 is a 90-110 kD IL-10 receptor. It is a class II cytokine receptor expressed on thymocytes, T cells, B cells, NK cells, monocytes and macrophages. Ligand binding of CD210 induces Jak1 and Tyk, resulting in STAT1 and STAT3 activation. IL-10 receptor stimulation results in the inhibition of cytokine production and the costimulation of B cell proliferation and differentiation. The only known ligand for this receptor is IL-10.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Soluble mIL-10R-FLAG-His\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of ligand binding1-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 112706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112704) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin \u0026lt; 0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 112710) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin \u0026lt; 0.01 EU\/µg).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): O'Farrell A, et al. 1998. EMBO J. 17:1006. (Block) Wang W, et al. 2004. FASEB J. 18:1043. (Block) Vieira PL, et al. 2004. J. Immunol. 172:5986. (Block) Iwashiro M, et al. 2001. P. Natl. Acad. Sci. USA 98:9226. (Block) Vicari AP, et al. 2002. J. Exp. Med. 196:541. (Block)\u003cbr\u003e\nProduct Citations: Bhattacharyya A, et al. 2022. Am J Physiol Lung Cell Mol Physiol. 322:L495. PubMed Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed Castiglioni A, et al. 2015. PLoS One. 10:128094. PubMed Lv H, et al. 2020. Cell Metabolism. 33(1):110-127.e5. PubMed Matsushita T, et al. 2010. J Immunol. 185:2240. PubMed Yoon S, et al. 2009. J Leukoc Biol. 86:1311. PubMed Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed Hu Z, et al. 2013. J Immunol. 191:312. PubMed Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed\u003cbr\u003e\nRRID: AB_313518 (BioLegend Cat. No. 112705) AB_313519 (BioLegend Cat. No. 112706)\u003cbr\u003e\nStructure: Class II cytokine receptor family, 90-110 kD\u003cbr\u003e\nDistribution: Thymocytes, T and B cells, NK cells, monocytes, macrophages\u003cbr\u003e\nFunction: Inhibits cytokine production, costimulates B cell proliferation and differentiation\u003cbr\u003e\nLigand\/Receptor: IL-10\u003cbr\u003e\nCell Type: B cells, Macrophages, Monocytes, NK cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Ho A, et al. 1993. P. Natl. Acad. Sci. USA 90:11267. 2. Tan JC, et al. 1993. J. Biol. Chem. 268:21053. 3. Niemand C, et al. 2003. J. Immunol. 170:3263. 4. Corinti S, et al. 2001. J. Immunol. 166:4312.\u003cbr\u003e\nGene ID: 16154\u003cbr\u003e\nUniProt: View information about CD210 on UniProt.org\u003cbr\u003e\nClone: 1B1.3a\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL-10R, Interleukin-10 Receptor\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860841287849,"sku":"112705","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-112705","provider":"Iright","version":"1.0","type":"link"}