{"product_id":"biolegend-112904","title":"Biolegend, 112904, Biotin anti-mouse CD120a (TNF R Type I\/p55) Antibody, 500μg","description":"\u003cp\u003eCD120a is a 55 kD Type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type I (TNFRI) or p55. It is expressed on a variety of cells at low levels. Resting T cells and erythrocytes express very little to no CD120a. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRADD and RIP, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state. The 55R-170 antibody has been shown to block in vitro and in vivo receptor signaling initiated by ligand binding.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: E. coli-expressed extracellular domain of the mouse TNFRI protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality testedELISA Detection - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Flow Cytometry: For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112904) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).ELISA Detection1: The biotinylated 55R-170 antibody is useful as a detection antibody when paired with the Purified 55R-286 antibody (Cat. No. 113002) as the capture antibody.Additional reported applications (for the relevant formats) include:immunoprecipitation1, and In vitro and In vivo receptor blocking1. The Ultra-LEAF™Purified antibody (Endotoxin \u0026lt;0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 112905 \u0026amp; 112906).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Sheehan, K. C., et al. 1995. J. Exp. Med. 181:607.\u003cbr\u003e\nRRID: AB_313528 (BioLegend Cat. No. 112903) AB_313529 (BioLegend Cat. No. 112904)\u003cbr\u003e\nStructure: TNFR superfamily, 55 kD\u003cbr\u003e\nDistribution: Variety of cell types at low levels\u003cbr\u003e\nFunction: Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation\u003cbr\u003e\nLigand\/Receptor: TNF-α, LT-α (TNF-β)\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Aggarwal, B. B., et al. 1985. Nature 318 665. 2. Chan, F. K.M., et al. 2000. Science 288:2351. 3. Loetscher, H., et al. 1990. Cell 61:351. 4. Rothe, J., et al. 1993. Nature 364:798.\u003cbr\u003e\nGene ID: 21937\u003cbr\u003e\nUniProt: View information about CD120a on UniProt.org\u003cbr\u003e\nClone: 55R-170\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862443741353,"sku":"112904","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-112904","provider":"Iright","version":"1.0","type":"link"}