{"product_id":"biolegend-114618","title":"Biolegend, 114618, APC\/Fire™ 750 anti-mouse H-2K, 100μg","description":"\u003cp\u003eThe 28-8-6 antibody reacts with the H-2K b and H-2D b MHC class I alloantigens expressed on nucleated cells from mice of the H-2K b \/H-2D b haplotype. H-2K b \/H-2D b is involved in antigen presentation to T cells expressing CD3\/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2D d MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: C3H.SW mouse splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC\/Fire™ 750 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC- Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.* APC\/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt) Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC) Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed. Shao H, et al. 2005. J. Immunol. 175:1851. (FC)\u003cbr\u003e\nProduct Citations: Rankin LC, et al. 2023. Cell Rep. 112135:42. PubMed Aghayev T, et al. 2022. Cancer Discov. 12:1960. PubMed Shukla A, et al. 2021. Adv Nanobiomed Res. 1:. PubMed\u003cbr\u003e\nRRID: AB_2750197 (BioLegend Cat. No. 114617) AB_2750198 (BioLegend Cat. No. 114618)\u003cbr\u003e\nStructure: MHC class I\u003cbr\u003e\nDistribution: Nucleated cells of the H-2Kb\/H-2Db haplotype\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD8\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Ozato K, et al. 1981. J. Immunol. 126:317. 2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447. 3. Evans GA, et al. 1992. Nature 300:755.\u003cbr\u003e\nGene ID: 1496414972\u003cbr\u003e\nUniProt: View information about H-2Kb\/H-2Db on UniProt.org\u003cbr\u003e\nClone: 28-8-6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class I\u003cbr\u003e\nIsotype: Mouse (C3H) IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860882870441,"sku":"114618","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-114618","provider":"Iright","version":"1.0","type":"link"}