{"product_id":"biolegend-114716","title":"Biolegend, 114716, PerCP\/Cyanine5.5 anti-mouse H-2K, 100μg","description":"\u003cp\u003eThe 34-1-2S antibody reacts with the H-2K d \/H-2D d MHC class I alloantigens expressed on nucleated cells from mice of the H-2K d \/H-2D d haplotype. H-2K d \/H-2D d is involved in antigen presentation to T cells expressing CD3\/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: BDF Splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2, complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ozato K, et al. 1982. Transplantation 34:113. Sester M, et al. 2000. J. Biol. Chem. 34:113.\u003cbr\u003e\nProduct Citations: Li X, et al. 2022. Nat Commun. 13:2794. PubMed\u003cbr\u003e\nRRID: AB_2734175 (BioLegend Cat. No. 114715) AB_2734176 (BioLegend Cat. No. 114716)\u003cbr\u003e\nStructure: MHC class I\u003cbr\u003e\nDistribution: Nucleated cells of the H-2Kd\/H-2Dd haplotype\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD8\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: Watts C. 1997. Ann. Rev. Immunol. 15:821. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.\u003cbr\u003e\nGene ID: 1496414972\u003cbr\u003e\nUniProt: View information about H-2Kd\/H-2Dd on UniProt.org\u003cbr\u003e\nClone: 34-1-2S\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC Class I\u003cbr\u003e\nIsotype: Mouse (C3H) IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862374797481,"sku":"114716","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-114716","provider":"Iright","version":"1.0","type":"link"}