{"product_id":"biolegend-115553","title":"Biolegend, 115553, PE\/Dazzle™ 594 anti-mouse CD19 Antibody, 25μg","description":"\u003cp\u003eCD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse CD19-expressing K562 human erythroleukemia cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Dazzle™ 594 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE\/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence7.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC) Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed Olive V, et al. 2013. Elife. 2:822. PubMed Miyai T, et al. 2014. PNAS. 111:11780. PubMed\u003cbr\u003e\nProduct Citations: Rocca Y, et al. 2023. Methods Mol Biol. 2618:187. PubMed Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed Wang Z, et al. 2023. J Cancer. 14:1049. PubMed Jebbawi F, et al. 2021. Parasite Immunol. 43:e12834. PubMed van Dierendonck XAMH, et al. 2022. Proc Natl Acad Sci U S A. 119:e2114739119. PubMed Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed Lees JG, et al. 2020. PLoS One. 15:e0238164. PubMed Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed Geng T, et al. 2022. Methods Mol Biol. 2585:71. PubMed Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed Baier FA, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:745. PubMed França TT, et al. 2021. JCI Insight. 6:. PubMed Kuhn JA, et al. 2021. Elife. 10:. PubMed Lingegowda H, et al. 2022. Front Reprod Health. 3:726936. PubMed Yeung F, et al. 2020. Cell Host \u0026amp; Microbe. 27(5):809-822. PubMed Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed\u003cbr\u003e\nRRID: AB_2564000 (BioLegend Cat. No. 115553) AB_2564001 (BioLegend Cat. No. 115554)\u003cbr\u003e\nStructure: Ig superfamily, associates with CD21 and CD81, 95 kD\u003cbr\u003e\nDistribution: Pro-B cells to mature B cells (during development), follicular dendritic cells\u003cbr\u003e\nFunction: Modulates B cell activation and differentiation\u003cbr\u003e\nLigand\/Receptor: CD21, CD81, Leu-13\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.\u003cbr\u003e\nGene ID: 12478\u003cbr\u003e\nUniProt: View information about CD19 on UniProt.org\u003cbr\u003e\nClone: 6D5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B4\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862476509353,"sku":"115553","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-115553","provider":"Iright","version":"1.0","type":"link"}