{"product_id":"biolegend-116108","title":"Biolegend, 116108, PE anti-mouse CD54 Antibody, 200μg","description":"\u003cp\u003eCD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes\/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a\/CD18) and Mac-1 (CD11b\/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1\/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse NS-1 cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3 of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 116131-116136).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kumaska T, et al. 1996. J. Clin. Invest. 97:2362. (Block) Horley KJ, et al. 1989. EMBO J. 8:2889. (Block) Burns AR, et al. 1994. J. Immunol. 153:3189. (IHC) Takei F, et al. 1985. J. Immunol. 134:1403. (Block, IP) Sumagin R, et al. 2010. J. Immunol. 184:5242. PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Seidler DG, et al. 2011 J. Immunol. 187:6108. PubMed Chacko AM, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PUbMed El-Assaad F, et al. 2013. Infect Immun. 81:3984. PubMed Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed\u003cbr\u003e\nProduct Citations: Brenner JS, et al. 2018. Nat Commun. 9:20222. PubMed Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed Cayre S, et al. 2020. Development. 147:00:00. PubMed Kitur K, et al. 2016. Cell Rep. 16:2219-2230. PubMed Chao TL, et al. 2020. Front Microbiol. 2.459722222. PubMed Dúcka M, et al. 2021. Neoplasia. 23:326. PubMed Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed Kobori T, et al. 2018. Front Immunol. 9:334. PubMed Lachenmaier S, et al. 2011. J Neuroimmunol. 232:119. PubMed Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed Wang Y, et al. 2021. Nat Commun. 12:2343. PubMed Bresson L, et al. 2018. Development. 145. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Moretti FA, et al. 2018. Elife. 7:e35816. PubMed Ise W, et al. 2018. Immunity. 48:702. PubMed Chiche A, et al. 2019. Breast Cancer Res. 21:13. PubMed Guilliams M, et al. 2022. Cell. 185:379. PubMed Ponzetta A, et al. 2020. Cell. 178(2):346-360.e24.. PubMed Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed Bordas M, et al. 2020. Int J Mol Sci. 21:00. PubMed Elgazar-Carmon V, et al. 2008. J Lipid Res. 49:1849. PubMed van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed Korin B, et al. 2020. Sleep. :43. PubMed Corbet M, et al. 2021. PLoS Pathog. 17:e1010069. PubMed Cayre S, et al. 2020. Development. . PubMed Thomson CA, et al. 2018. J Immunol. 201:215. PubMed Zhong J, et al. 2021. Commun Biol. 4:865. PubMed\u003cbr\u003e\nRRID: AB_313698 (BioLegend Cat. No. 116107) AB_313699 (BioLegend Cat. No. 116108)\u003cbr\u003e\nStructure: Ig superfamily, 90 kD\u003cbr\u003e\nDistribution: Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes\/macrophages, granulocytes, dendritic cells\u003cbr\u003e\nFunction: Immune reaction, inflammation, adhesion\u003cbr\u003e\nLigand\/Receptor: CD11a\/CD18 (LFA-1) or CD11b\/CD18 (Mac-1) and CD11c\/CD18, CD43, hyaluronan, fibrinogen\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Springer TA. 1990. Nature 346:425. 3. Rothlein R, et al. 1986. J. Immunol. 137:1270.\u003cbr\u003e\nGene ID: 15894\u003cbr\u003e\nUniProt: View information about CD54 on UniProt.org\u003cbr\u003e\nClone: YN1\/1.7.4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: ICAM-1, Ly-47\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864106619049,"sku":"116108","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-116108","provider":"Iright","version":"1.0","type":"link"}