{"product_id":"biolegend-116123","title":"Biolegend, 116123, PerCP\/Cyanine5.5 anti-mouse CD54 Antibody, 25μg","description":"\u003cp\u003eCD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes\/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a\/CD18) and Mac-1 (CD11b\/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1\/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse NS-1 cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3 of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 116131-116136).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kumaska T, et al. 1996. J. Clin. Invest. 97:2362. (Block) Horley KJ, et al. 1989. EMBO J. 8:2889. (Block) Burns AR, et al. 1994. J. Immunol. 153:3189. (IHC) Takei F, et al. 1985. J. Immunol. 134:1403. (Block, IP) Sumagin R, et al. 2010. J. Immunol. 184:5242. PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Seidler DG, et al. 2011 J. Immunol. 187:6108. PubMed Chacko AM, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PUbMed El-Assaad F, et al. 2013. Infect Immun. 81:3984. PubMed Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed\u003cbr\u003e\nProduct Citations: Béguelin W, et al. 2020. Cancer Cell. 37(5):655-673. PubMed Shue YT, et al. 2022. Nat Commun. 13:2690. PubMed\u003cbr\u003e\nRRID: AB_2715951 (BioLegend Cat. No. 116123) AB_2715952 (BioLegend Cat. No. 116124)\u003cbr\u003e\nStructure: Ig superfamily, 90 kD\u003cbr\u003e\nDistribution: Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes\/macrophages, granulocytes, dendritic cells\u003cbr\u003e\nFunction: Immune reaction, inflammation, adhesion\u003cbr\u003e\nLigand\/Receptor: CD11a\/CD18 (LFA-1) or CD11b\/CD18 (Mac-1) and CD11c\/CD18, CD43, hyaluronan, fibrinogen\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Springer TA. 1990. Nature 346:425. 3. Rothlein R, et al. 1986. J. Immunol. 137:1270.\u003cbr\u003e\nGene ID: 15894\u003cbr\u003e\nUniProt: View information about CD54 on UniProt.org\u003cbr\u003e\nClone: YN1\/1.7.4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: ICAM-1, Ly-47\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864106881193,"sku":"116123","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-116123","provider":"Iright","version":"1.0","type":"link"}