{"product_id":"biolegend-116510","title":"Biolegend, 116510, Alexa Fluor® 488 anti-mouse H-2K, 100μg","description":"\u003cp\u003eThe AF6-88.5 antibody reacts with H-2K b MHC class I alloantigen expressed on nucleated cells from mice of the H-2K b haplotype. H-2K b is involved in antigen presentation to T cells expressing CD3\/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: C57BL mouse splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections. Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Loken MR, et al. 1982. J. Immunol. Methods 50:R85. Wall KA, et al. 1983. J. Immunol. 131:1056. Andersson M, et al. 1998. J. Immunol. 161:6475. Shao H, et al. 2005. J. Immunol. 175:1851. Hui S, et al. 2005. J. Immunol. 175:1851. PubMed Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed\u003cbr\u003e\nProduct Citations: Balakrishnan PB, et al. 2022. Nano Res. 15:2300. PubMed Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed Tsurutani N, et al. 2016. J Immunol. 196: 124 - 134. PubMed\u003cbr\u003e\nRRID: AB_492915 (BioLegend Cat. No. 116510)\u003cbr\u003e\nStructure: MHC class I\u003cbr\u003e\nDistribution: Nucleated cells of the H-2Kb haplotype\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD8\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Watts C. 1997. Ann. Review Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.\u003cbr\u003e\nGene ID: 14972\u003cbr\u003e\nUniProt: View information about H-2Kb on UniProt.org\u003cbr\u003e\nClone: AF6-88.5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class I\u003cbr\u003e\nIsotype: Mouse (BALB\/c) IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862535098537,"sku":"116510","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-116510","provider":"Iright","version":"1.0","type":"link"}