{"product_id":"biolegend-116809","title":"Biolegend, 116809, Pacific Blue™ anti-mouse Ly-49A Antibody, 25μg","description":"\u003cp\u003eLy-49A is a 44 kD member of the C-type lectin\/Ly-49 family proteins. It is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Ly-49A is present on a subset of NK and NK-T cells. Ly-49A, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. The Ly-49A receptor recognizes H-2D d and H-2D k , triggering phosphorylation of the ITIM motif in the cytoplasmic domain and recruitment of SHP-1 and SHP-2. The YE1\/48.10.6 antibody reacts with NK cells from all mouse strains tested and reverses the inhibition of NK cytotoxicity.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Con A activated splenic T cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,2.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Takei F. 1983. J. Immunol. 130:2794. Chan P, et al. 1989. J. Immunol. 142:1727.\u003cbr\u003e\nProduct Citations: Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed Yang E, et al. 2016. J Immunol. 197: 934 - 941. PubMed Wagner AK, et al. 2022. iScience. 25:105137. PubMed Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed\u003cbr\u003e\nRRID: AB_572013 (BioLegend Cat. No. 116810)\u003cbr\u003e\nStructure: C-type lectin, Ly-49, ITIM, 44 kD\u003cbr\u003e\nDistribution: NK and NK-T cells\u003cbr\u003e\nFunction: Inhibitory NK cell receptor\u003cbr\u003e\nLigand\/Receptor: MHC Class I Molecules\u003cbr\u003e\nCell Type: NK cells, NKT cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nAntigen References: 1. Coles MC, et al. 2000. Eur. J. Immunol. 30:236. 2. Takei FJ, et al. 1997. Immunol. Rev. 155:67. 3. Hanke T, et al. 1999. Immunity 11:67.\u003cbr\u003e\nGene ID: 16627\u003cbr\u003e\nUniProt: View information about Ly-49A on UniProt.org\u003cbr\u003e\nClone: YE1\/48.10.6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Klra1, A1, killer cell lectin-like receptor, subfamily A, member 1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864105210025,"sku":"116809","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-116809","provider":"Iright","version":"1.0","type":"link"}