{"product_id":"biolegend-120608","title":"Biolegend, 120608, APC anti-mouse LPAM-1 (Integrin α4β7) Antibody, 100μg","description":"\u003cp\u003eDATK32 antibody is specific for a combinatorial determinate of integrin α4β7complex. Integrin α4β7 is composed of a 150 kD (α4 or CD49d) and a 130 kD (β7) heterodimer, also known as CD49d\/β7 or LPAM-1. Belonging to the Ig superfamily, it is found on the majority of peripheral lymphocytes and subsets of thymocytes and bone marrow cells (including mast cell progenitors). Integrin α4β7 binds its ligands, VCAM-1 (CD106), MAdCAM-1 and fibronectin, and plays an important role in lymphocytes adhesion and the direction of migration of blood lymphocytes to the intestine and associated lymphoid tissues.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: TK1 cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell adhesion1-4, in vitro induction of TK1 cell aggregation1, immunoprecipitation4, and immunohistochemical staining of frozen sections5. It has been reported that the DATK32 mAb is able to block a4ß7 mediated lymphocyte adhesion to VCAM-1, MAdCAM-1, and fibronectin in vitro and in vivo.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block) Berlin C, et al. 1993. Cell 74:185. (Block) Berlin-Rufenach C, et al. 1999. J. Exp. Med. 189:1467. (Block) Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block, IP) Bogetto L, et al. 2000. Blood 95:2397. (IHC)\u003cbr\u003e\nProduct Citations: Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed Kawano H, et al. 2016. Int Immunol. 28: 489 - 501. PubMed Tian Y, et al. 2016. J Immunol. 196: 2153 - 2166. PubMed Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed Knolle MD et al. 2018. Frontiers in immunology. 1.925 . PubMed Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed Ghaedi M, et al. 2020. The Journal of Experimental Medicine. 217(3):jem. PubMed Kitamoto S, et al. 2020. Cell. 182(2):447-462.e14. PubMed\u003cbr\u003e\nRRID: AB_10719833 (BioLegend Cat. No. 120607) AB_10730607 (BioLegend Cat. No. 120608)\u003cbr\u003e\nStructure: Integrin family, Ig superfamily, 150 kD\/130 kD heterodimer\u003cbr\u003e\nDistribution: Majority of peripheral lymphocytes, subsets of thymocytes and bone marrow cells\u003cbr\u003e\nFunction: Lymphocyte adhesion\u003cbr\u003e\nLigand\/Receptor: VCAM-1 (CD106), MAdCAM-1, fibronectin\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Andrew DP, et al. 1994. J. Immunol. 153:3847. 2. Berlin C, et al. 1994. Cell 74:185. 3. Gurish MF, et al. 2001 J. Exp. Med. 194:1243. 4. Hamann A, et al. 1994. J. Immunol. 152:3282.\u003cbr\u003e\nGene ID: 31114425713\u003cbr\u003e\nUniProt: View information about LPAM-1 on UniProt.org\u003cbr\u003e\nClone: DATK32\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: α4β7 Integrin, CD49d\/β7, LPAM-1, ITGA4, ITGB7\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863005450409,"sku":"120608","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-120608","provider":"Iright","version":"1.0","type":"link"}