{"product_id":"biolegend-121008","title":"Biolegend, 121008, PerCP\/Cyanine5.5 anti-human\/mouse integrin β7 Antibody, 100μg","description":"\u003cp\u003eIntegrin β7 is a 130 kD glycoprotein, also known as integin βp. It is a member of the Ig superfamily. In association with integrin α4 or αE chain, β7 forms α4\/β7 or αE\/β7 heterodimer. α4\/β7 (CD49d\/β7, LPAM-1) is expressed on majority of peripheral lymphocytes, small subsets of thymocytes and bone marrow progenitors. LPAM-1 binds to several ligands, VCAM-1, MAdCAM-1 and fibronectin, and is involved in lymphocyte adhesion, some hematopoietic progenitor cells migration. αE\/β7 (CD103\/β7,α IEL \/β7) is expressed on intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, T regulatory cells, a subset of CD8+ T cells in lymph nodes and lamina propria. CD103\/β7 complex is thought to play a role in lymphocyte retention via interaction with its lignd E-Cadherin. The FIB27 antibody has been reported to react with mouse and human β7 integrin and to block β7 integrin-mediated cell adhesion in in vitro and in vivo studies.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse, Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: TK1 cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block) Berlin C, et al. 1993. Cell 74:185. (Block) Tidswell M, et al. 1997. J. Immunol. 159:1497. (Block)\u003cbr\u003e\nProduct Citations: Fischer A, et al. 2015. Gut. 101136\/gutjnl-2015-310022. PubMed Piotrowski J, et al. 2013. Mol Cell Biol. 33:958. PubMed Port JR, et al. 2020. J Virol. 94:. PubMed Kanemaru K, et al. 2015. PLoS One. 10: 0144436. PubMed\u003cbr\u003e\nRRID: AB_1659234 (BioLegend Cat. No. 121007) AB_2129312 (BioLegend Cat. No. 121008)\u003cbr\u003e\nStructure: Integrin family, Ig superfamily, 130 kD, heterodimer with CD49d or CD103\u003cbr\u003e\nDistribution: CD49d\/ß7: majority of lymphocytes, thymocyte subset, and some bone marrow progenitors; CD103\/ß7: intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, subset of CD8 T cells in lymph nodes\u003cbr\u003e\nFunction: Lymphocyte adhesion, hematopoietic progenitor cells migration\u003cbr\u003e\nLigand\/Receptor: CD49d\/ß7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103\/ß7: E-cadherin\u003cbr\u003e\nCell Type: Hematopoietic stem and progenitors, Lymphocytes, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Andrew DP, et al. 1994. J. Immunol. 153:3847. 2. Picarella D, et al. 1997. J. Immunol. 158:2099. 3. Lefrancois L, et al. 1994. Eur. J. Immunol. 24:635 4. Cepek KL, et al. 1994. Nature 372:190.\u003cbr\u003e\nGene ID: 164213695\u003cbr\u003e\nUniProt: View information about Integrin beta7 on UniProt.org\u003cbr\u003e\nClone: FIB27\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: β7 Integrin, integrin βp, ITGB7\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864001106089,"sku":"121008","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-121008","provider":"Iright","version":"1.0","type":"link"}