{"product_id":"biolegend-121413","title":"Biolegend, 121413, APC anti-mouse CD103 Antibody, 25μg","description":"\u003cp\u003eCD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin α IEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β 7 , CD103 is expressed as αE\/β 7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: Mouse intestinal intraepithelial lymphocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,7 of acetone-fixed frozen sections, immunofluorescence2, and in vitro activation1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP) Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF) Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)\u003cbr\u003e\nProduct Citations: Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed Zheng X, et al. 2019. PLoS Pathog. 15:e1008036. PubMed Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed Peng C, et al. 2022. Immunity. 55:98. PubMed Liu L, et al. 2022. Nat Commun. 13:6740. PubMed Fiege JK, et al. 2021. Cell Host Microbe. 29:1815. PubMed Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed Ren Z, et al. 2021. EMBO Molecular Medicine. :e14059. PubMed Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed Dong L, et al. 2021. Cancer Cell. . PubMed Barry KC, et al. 2018. Nat Med. 24:1178. PubMed Aldon Y, et al. 2020. J Immunol. 204:903. PubMed Sng XYX, et al. 2020. J Immunol. 204:3108. PubMed Wu J, et al. 2020. Cell Reports. 31(1):107484. PubMed Li Y, et al. 2020. Cell Rep. 30:1753. PubMed Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed Okamoto T, et al. 2020. Cancer Res. 3580:80. PubMed Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed Wu L, et al. 2022. Theranostics. 12:842. PubMed Cassidy BR, et al. 2020. J Neuroinflammation. 17:259. PubMed Gozgit JM, et al. 2021. Cancer Cell. :. PubMed Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed Tavazoie MF, et al. 2018. Cell. 172:825. PubMed Place D, et al. 2017. PLoS One. 10.1371\/journal.pone.0190384. PubMed Gabriel SS, et al. 2021. Immunity. 54(8):1698-1714.e5. PubMed Jin C, et al. 2019. Cell. 176:998. PubMed Nam GH, et al. 2018. Nat Commun. 9:2165. PubMed Jin C, et al. 2022. Oncoimmunology. 11:2099642. PubMed Yamada D, et al. 2014. J Immunol. 192:4112. PubMed Morikawa M, et al. 2016. PLoS One. 11:e0163607. PubMed Kim M, et al. 2018. Immunity. 49:151. PubMed Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Xiao Y, et al. 2021. Cell. 184:6037. PubMed Stefan KL, et al. 2020. Cell. 1312:183. PubMed Bonavita E, et al. 2020. Immunity. 1215:53. PubMed Li J, et al. 2021. Cell Reports. 34(11):108839. PubMed Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed Wu L, et al. 2020. Cancer Immunol Res. 710:8. PubMed Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed Chng S, et al. 2016. Sci Rep. 6: 23820. PubMed Paiva C, et al. 2010. Rheumatology. 49:246. PubMed Riopel M, et al. 2019. Mol Metab. 20:89. PubMed Pan Y, et al. 2021. NPJ Vaccines. 6:1. PubMed\u003cbr\u003e\nRRID: AB_1227503 (BioLegend Cat. No. 121413) AB_1227502 (BioLegend Cat. No. 121414)\u003cbr\u003e\nStructure: Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin\u003cbr\u003e\nDistribution: Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.\u003cbr\u003e\nFunction: Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.\u003cbr\u003e\nLigand\/Receptor: E-Cadherin\u003cbr\u003e\nCell Type: Dendritic cells, Lymphocytes, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201. 2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852. 3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635. 4. Sung SS, et al. 2006. J. Immunol. 176:2161. 5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063. 6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.\u003cbr\u003e\nGene ID: 16407\u003cbr\u003e\nUniProt: View information about CD103 on UniProt.org\u003cbr\u003e\nClone: 2E7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Integrin αIEL chain, Integrin αE chain, αE integrin, ITGAE\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862320468137,"sku":"121413","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-121413","provider":"Iright","version":"1.0","type":"link"}