{"product_id":"biolegend-122511","title":"Biolegend, 122511, APC anti-mouse Ly-6A\/E (Sca-1) Antibody, 25μg","description":"\u003cp\u003eLy-6A\/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A\/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA\/2, SJL, SWR, and 129), Ly-6A\/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB\/c, CBA, C3H\/He, DBA\/1, and NZB) have low Ly-6A\/E expression on resting peripheral lymphocytes. The expression of Ly-6A\/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A\/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: mouse pre-T cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD) Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC) van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP) van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF) Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. Artinger EL, et al. 2013. PNAS. 110:12000. PubMed\u003cbr\u003e\nProduct Citations: Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed Issa N, et al. 2023. Leukemia. :. PubMed Almada AE, et al. 2021. Cell Reports. 34(4):108656. PubMed Xie T, et al. 2021. Sci Adv. 7: . PubMed Karra L, et al. 2020. Oncogene. 6920:39. PubMed Bultmann–Mellin I, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 313:L687. PubMed Britschgi A, et al. 2017. Nature. 541:541-545. PubMed Masschelein E, et al. 2020. Skelet Muscle. 10:21. PubMed Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed Lopes C, et al. 2016. PLoS One. 11: 0162927. PubMed Simon M, et al. 2019. Cell Metab. 29:871. PubMed Ogawa-Wong A, et al. 2022. Metabolites. 12:. PubMed Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed Majumdar D, et al. 2021. Curr Protoc. 1:e216. PubMed Celik H, et al. 2018. Cancer Cell. 34:741. PubMed Carmody C et al. 2019. Endocrinology. 160(5):1205-1222 . PubMed Siddique SM, et al. 2019. Sci Rep. 9:13977. PubMed Sakai H, et al. 2022. iScience. 25:103928. PubMed Wang X, et al. 2018. Nat Commun. 9:551. PubMed An X, et al. 2020. Thyroid. . PubMed\u003cbr\u003e\nRRID: AB_756196 (BioLegend Cat. No. 122511) AB_756197 (BioLegend Cat. No. 122512)\u003cbr\u003e\nStructure: Ly-6 multigene family, 18 kD\u003cbr\u003e\nDistribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells\u003cbr\u003e\nFunction: Regulates B and T cell responses\u003cbr\u003e\nCell Type: B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells\u003cbr\u003e\nBiology Area: Immunology, Stem Cells\u003cbr\u003e\nAntigen References: 1. Rock KL, et al. 1989. Immunol. Rev. 111:195. 2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709.\u003cbr\u003e\nGene ID: 110454\u003cbr\u003e\nUniProt: View information about Ly-6A\/E on UniProt.org\u003cbr\u003e\nClone: E13-161.7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Sca-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866087510185,"sku":"122511","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-122511","provider":"Iright","version":"1.0","type":"link"}