{"product_id":"biolegend-123121","title":"Biolegend, 123121, Alexa Fluor® 647 anti-mouse F4\/80 Antibody, 25μg","description":"\u003cp\u003eF4\/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4\/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4\/80 has not been identified, but it has been reported that F4\/80 is required for the induction of CD8 + T cells-mediated peripheral tolerance.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Murine macrophages\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F, 3D IHC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10 µg\/mL. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC) Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC) Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC) Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC) Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed Watson NB, et al. 2015. J Immunol. 194:2796. PubMed Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Marneros AG, et al. 2020. Developmental Cell. 54(3):348-366.e5. PubMed Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed Barkaway A, et al. 2021. Immunity. . PubMed Palacios-Arreola M, et al. 2017. Sci Rep. 10.1038\/s41598-017-10135-1. PubMed Ferriz M, et al. 2023. STAR Protoc. 4:102079. PubMed Obi AT, et al. 2023. Sci Rep. 13:3253. PubMed Bautista CA, et al. 2023. Front Physiol. 14:1122348. PubMed Lütge M, et al. 2023. Nat Immunol. . PubMed Doloff JC, et al. 2023. Sci Adv. 9:eade9488. PubMed Yan M, et al. 2022. J Cell Physiol. 237:897. PubMed Lees-Shepard JB, et al. 2022. J Clin Invest. 132: . PubMed Wang L, et al. 2022. Cell Rep. 41:111462. PubMed Aljobaily N, et al. 2022. 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PubMed Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed\u003cbr\u003e\nRRID: AB_893492 (BioLegend Cat. No. 123121) AB_893480 (BioLegend Cat. No. 123122)\u003cbr\u003e\nStructure: EGF-TM7 family member, 160 kD glycoprotein\u003cbr\u003e\nDistribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells\u003cbr\u003e\nFunction: Induction of immunological tolerance\u003cbr\u003e\nCell Type: Dendritic cells, Langerhans cells, Macrophages, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nAntigen References: 1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805. 2. Hume DA, et al. 1983. J. Exp. Med. 158:1522. 3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801. 4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486. 5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.\u003cbr\u003e\nGene ID: 13733\u003cbr\u003e\nUniProt: View information about F4\/80 on UniProt.org\u003cbr\u003e\nClone: BM8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: EMR1, Ly71\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862912880809,"sku":"123121","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-123121","provider":"Iright","version":"1.0","type":"link"}