{"product_id":"biolegend-123505","title":"Biolegend, 123505, Biotin anti-mouse CD1d (CD1.1, Ly-38) Antibody, 50μg","description":"\u003cp\u003eCD1d, also known as CD1.1 and Ly-38, is a 48 kD type I membrane glycoprotein that is structurally similar to MHC class I and is non-covalently associated with β2-microglobulin. In humans, the CD1 family consists of group I (CD1a, CD1b, and CD1c), group II (CD1d), and group III (CD1e) proteins, but CD1d is the only CD1 molecule found in mice. Mouse CD1d has broad tissue distribution, and is found on leukocytes, dendritic cells, epithelial cells, and thymocytes. CD1d plays a role in presenting non-peptide glycolipid antigens to CD1d-restricted T cells, and PKCδ has been identified as a critical regulator of CD1d-mediated antigen presentation.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse CD1.1 cDNA-transfected RMA-S mouse T lymphoma\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality testedIHC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistical staining, and blocking function3. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Fischer K, et al. 2004. P. Natl. Acad. Sci. USA 101:10685. (Block) Brozovic S, et al. 2004. Nat. Med. 10:535. Brossay L, et al. 1997. J. Immunol.. 159:1216. (Block) Jiang J, et al. 2012. PLoS One. 7:47487. PubMed\u003cbr\u003e\nProduct Citations: Kolan S, et al. 2015. Biochem Biophys Res Commun. 460:645. PubMed Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed Rohrbeck L, et al. 2020. Immunol Cell Biol. . PubMed\u003cbr\u003e\nRRID: AB_1236543 (BioLegend Cat. No. 123505) AB_1236548 (BioLegend Cat. No. 123506)\u003cbr\u003e\nStructure: 48 kD glycoprotein, Ig superfamily\u003cbr\u003e\nDistribution: leukocytes, dendritic cells, epithelial cells, thymocytes\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: similar to MHC class I, associate with ß-microglobulin\u003cbr\u003e\nCell Type: Dendritic cells, Epithelial cells, Leukocytes, Thymocytes\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Brudin N, et al. 1998. J. Immunol. 161:3271. 2. Amano M, et al. 1998. J. Immunol. 161:1710. 3. Brossay L, et al. 1997. J. Immunol. 159:1216. 4. Dougan SK, et al. 2007. Curr. Top. Microbiol. Immunol. 314:113. 5. Brutkiewicz RR, et al. 2007. Eur. J. Immunol. 37:2390.\u003cbr\u003e\nGene ID: 12479\u003cbr\u003e\nUniProt: View information about CD1d on UniProt.org\u003cbr\u003e\nClone: 1B1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD1, CD1.1, Ly-38\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860839518377,"sku":"123505","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-123505","provider":"Iright","version":"1.0","type":"link"}