{"product_id":"biolegend-123527","title":"Biolegend, 123527, Brilliant Violet 421™ anti-mouse CD1d (CD1.1, Ly-38) Antibody, 50μg","description":"\u003cp\u003eCD1d, also known as CD1.1 and Ly-38, is a 48 kD type I membrane glycoprotein that is structurally similar to MHC class I and is non-covalently associated with β2-microglobulin. In humans, the CD1 family consists of group I (CD1a, CD1b, and CD1c), group II (CD1d), and group III (CD1e) proteins, but CD1d is the only CD1 molecule found in mice. Mouse CD1d has broad tissue distribution, and is found on leukocytes, dendritic cells, epithelial cells, and thymocytes. CD1d plays a role in presenting non-peptide glycolipid antigens to CD1d-restricted T cells, and PKCδ has been identified as a critical regulator of CD1d-mediated antigen presentation.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse CD1.1 cDNA-transfected RMA-S mouse T lymphoma\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistical staining, and blocking function3. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Fischer K, et al. 2004. P. Natl. Acad. Sci. USA 101:10685. (Block) Brozovic S, et al. 2004. Nat. Med. 10:535. Brossay L, et al. 1997. J. Immunol.. 159:1216. (Block) Jiang J, et al. 2012. PLoS One. 7:47487. PubMed\u003cbr\u003e\nProduct Citations: Liu J, et al. 2021. Front Immunol. 12:747730. PubMed\u003cbr\u003e\nRRID: AB_2800592 (BioLegend Cat. No. 123527)\u003cbr\u003e\nStructure: 48 kD glycoprotein, Ig superfamily\u003cbr\u003e\nDistribution: leukocytes, dendritic cells, epithelial cells, thymocytes\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: similar to MHC class I, associate with ß-microglobulin\u003cbr\u003e\nCell Type: Dendritic cells, Epithelial cells, Leukocytes, Thymocytes\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Brudin N, et al. 1998. J. Immunol. 161:3271. 2. Amano M, et al. 1998. J. Immunol. 161:1710. 3. Brossay L, et al. 1997. J. Immunol. 159:1216. 4. Dougan SK, et al. 2007. Curr. Top. Microbiol. Immunol. 314:113. 5. Brutkiewicz RR, et al. 2007. Eur. J. Immunol. 37:2390.\u003cbr\u003e\nGene ID: 12479\u003cbr\u003e\nUniProt: View information about CD1d on UniProt.org\u003cbr\u003e\nClone: 1B1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD1, CD1.1, Ly-38\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860840829097,"sku":"123527","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-123527","provider":"Iright","version":"1.0","type":"link"}