{"product_id":"biolegend-124611","title":"Biolegend, 124611, APC anti-mouse CD40 Antibody, 25μg","description":"\u003cp\u003eCD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34 + hematopoietic progenitors. CD40 regulates B cell development\/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant mouse CD40 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124627 \u0026amp;124628) with a low endotoxin limit (Endotoxin \u0026lt; 0.01 EU\/µg).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hasbold J, et al. 1994. Eur. J. Immunol. 24:1835. Bourgeois C, et al. 2002. Science 297:2060.\u003cbr\u003e\nProduct Citations: Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed Solier S, et al. 2023. Nature. 617:386. PubMed Doherty CM, et al. 2022. Parasite Immunol. 44:e12897. PubMed Motyka NI, et al. 2022. Infect Immun. 90:e0063721. PubMed Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371\/journal.pone.0182126. PubMed Kiritsy MC, et al. 2021. Elife. 10:. PubMed Chakrabarti J, et al. 2018. Oncotarget. 9:37439. PubMed Zhao J, et al. 2019. Nat Commun. 10:899. PubMed Jiang C, et al. 2020. Cell Reports. 28(5):1307-1322.e8.. PubMed Polavaram NS, et al. 2021. Bone Res. 9:24. PubMed Russler-Germain EV, et al. 2021. Elife. 10:. PubMed Guo Y, et al. 2022. Nat Commun. 13:6534. PubMed Farkas A, et al. 2013. J Immunol. 190:3319. PubMed Guo S, et al. 2021. Sci Rep. 11:23745. PubMed Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed Grabinger T, et al. 2019. Front Microbiol. 10:1385. PubMed Nakazawa Y, et al. 2021. Elife. 10:. PubMed Ren S, et al. 2022. Int J Biol Sci. 18:166. PubMed Mino T, et al. 2019. Nucleic Acids Res. 47:8838. PubMed Yang F, et al. 2021. Nat Commun. 12:3424. PubMed\u003cbr\u003e\nRRID: AB_1134081 (BioLegend Cat. No. 124611) AB_1134072 (BioLegend Cat. No. 124612)\u003cbr\u003e\nStructure: 48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.\u003cbr\u003e\nDistribution: Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.\u003cbr\u003e\nFunction: Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.\u003cbr\u003e\nLigand\/Receptor: CD154 on T cells\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, Dendritic cells, Macrophages\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.\u003cbr\u003e\nGene ID: 21939\u003cbr\u003e\nUniProt: View information about CD40 on UniProt.org\u003cbr\u003e\nClone: 3\/23\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Bp50, TNFRSF5\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862334656681,"sku":"124611","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-124611","provider":"Iright","version":"1.0","type":"link"}