{"product_id":"biolegend-124621","title":"Biolegend, 124621, PE\/Cyanine7 anti-mouse CD40 Antibody, 25μg","description":"\u003cp\u003eCD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34 + hematopoietic progenitors. CD40 regulates B cell development\/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant mouse CD40 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124627 \u0026amp;124628) with a low endotoxin limit (Endotoxin \u0026lt; 0.01 EU\/µg).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hasbold J, et al. 1994. Eur. J. Immunol. 24:1835. Bourgeois C, et al. 2002. Science 297:2060.\u003cbr\u003e\nProduct Citations: Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed Zhu J, et al. 2022. Research (Wash D C). 2022:9816272. PubMed Pierini S, et al. 2021. J Immunother Cancer. 9:. PubMed Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed Marangoni F, et al. 2021. Cell. . PubMed Huang YL, et al. 2015. Nat Commun. 6: 8141. PubMed Ciecko AE, et al. 2019. Cell Rep. 29:3073. PubMed Wu G, et al. 2020. Br J Pharmacol. 177:5063. PubMed Wang H, et al. 2019. Nat Commun. 10:1898. PubMed Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed Fontinha D, et al. 2015. PLoS One. 10: 0142540. PubMed Zhang J, et al. 2018. Sci Rep. 8:2373. PubMed Kramer K, et al. 2017. ACS Omega. 0.240972222. PubMed Elayeb R, et al. 2016. Haematologica. 101: 209 - 218. PubMed Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed Lu X, et al. 2019. Circ Res. 125:1055. PubMed Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed Zhang X, et al. 2021. Front Pharmacol. 12:706748. PubMed Chen R, et al. 2021. Front Oncol. 11:743050. PubMed Wang Y, et al. 2020. Vaccines (Basel). 8:. PubMed Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed Yang F, et al. 2021. Nat Commun. 12:3424. PubMed\u003cbr\u003e\nRRID: AB_10933422 (BioLegend Cat. No. 124621) AB_10897812 (BioLegend Cat. No. 124622)\u003cbr\u003e\nStructure: 48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.\u003cbr\u003e\nDistribution: Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.\u003cbr\u003e\nFunction: Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.\u003cbr\u003e\nLigand\/Receptor: CD154 on T cells\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, Dendritic cells, Macrophages\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.\u003cbr\u003e\nGene ID: 21939\u003cbr\u003e\nUniProt: View information about CD40 on UniProt.org\u003cbr\u003e\nClone: 3\/23\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Bp50, TNFRSF5\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862335279273,"sku":"124621","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-124621","provider":"Iright","version":"1.0","type":"link"}