{"product_id":"biolegend-124624","title":"Biolegend, 124624, PerCP\/Cyanine5.5 anti-mouse CD40 Antibody, 100μg","description":"\u003cp\u003eCD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34 + hematopoietic progenitors. CD40 regulates B cell development\/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant mouse CD40 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124627 \u0026amp;124628) with a low endotoxin limit (Endotoxin \u0026lt; 0.01 EU\/µg).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hasbold J, et al. 1994. Eur. J. Immunol. 24:1835. Bourgeois C, et al. 2002. Science 297:2060.\u003cbr\u003e\nProduct Citations: Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed Ivasko SM, et al. 2023. Front Immunol. 13:1023206. PubMed Gruber T, et al. 2020. JCI Insight. 5:00. PubMed Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed Francian A, et al. 2021. J Drug Target. 29:754. PubMed Samarchith P Kurup et al. 2019. Cell host \u0026amp; microbe. 25(4):565-577 . PubMed Ding X, et al. 2019. J Am Heart Assoc. 8:e011211. PubMed Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed Ravindran A, et al. 2014. Carcinogenesis. 35:959. PubMed Aglas L, et al. 2018. J Allergy Clin Immunol. 142:984. PubMed\u003cbr\u003e\nRRID: AB_2561473 (BioLegend Cat. No. 124623) AB_2561474 (BioLegend Cat. No. 124624)\u003cbr\u003e\nStructure: 48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.\u003cbr\u003e\nDistribution: Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.\u003cbr\u003e\nFunction: Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.\u003cbr\u003e\nLigand\/Receptor: CD154 on T cells\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, Dendritic cells, Macrophages\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.\u003cbr\u003e\nGene ID: 21939\u003cbr\u003e\nUniProt: View information about CD40 on UniProt.org\u003cbr\u003e\nClone: 3\/23\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Bp50, TNFRSF5\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862335344809,"sku":"124624","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-124624","provider":"Iright","version":"1.0","type":"link"}