{"product_id":"biolegend-126331","title":"Biolegend, 126331, Brilliant Violet 421™ anti-mouse CD357 (GITR) Antibody, 50μg","description":"\u003cp\u003eGITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF receptor superfamily, also known as TNFRSF18 and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD25 + CD4 + Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-κB activation, and abrogate the inhibitory function of CD25 + CD4 + Tregs. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse CD25+ CD4+ T cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): De Luca A, et al. 2007. J. Immunol. 179:5999. PubMed\u003cbr\u003e\nRRID: AB_3068231 (BioLegend Cat. No. 126331)\u003cbr\u003e\nStructure: 66-70 kD homodimer of TNFR superfamily member.\u003cbr\u003e\nDistribution: Low levels on resting T cells, B cells, and macrophages; high levels on CD25+ CD4+ Tregs and CD25+ CD4+ CD8- thymocytes.\u003cbr\u003e\nFunction: Stimulation of GITR can abrogate or reduce the inhibitory function of CD25+ CD4+ Treg cells. In vivo activation of GITR may lead to the development of autoimmune diseases.\u003cbr\u003e\nLigand\/Receptor: GITR ligand (GITRL).\u003cbr\u003e\nCell Type: B cells, Macrophages, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059. 2. Shimizu J, et al. 2002 Nat. Immunol. 3:135. 3. Stephens GL, et al. 2004. J. Immunol. 173:5008. 4. McHugh RS, et al. 2002. Immunity 16:311.\u003cbr\u003e\nRegulation: Activation of T and B cells can upregulate GITR expression.\u003cbr\u003e\nGene ID: 21936\u003cbr\u003e\nUniProt: View information about CD357 on UniProt.org\u003cbr\u003e\nClone: DTA-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Glucocorticoid-induced TNFR -related gene, TNFRSF18\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863030812841,"sku":"126331","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-126331","provider":"Iright","version":"1.0","type":"link"}