{"product_id":"biolegend-126505","title":"Biolegend, 126505, PE anti-mouse CD183 (CXCR3) Antibody, 25μg","description":"\u003cp\u003eCD183, also known as CXCR3, is a member of the C-X-C chemokine family, characterized by a pair of cysteine residues separated by a single amino acid. CXCR3 is a 38 kD seven pass transmembrane receptor coupled to G-protein. It mediates Ca 2+ mobilization and chemotaxis in response to C-X-C chemokines, such as IP10 (CXCL10), MIG (CXCL9), I-TAC (CXCL11) and PF4 (CXCL4). CXCR3 is expressed primarily on activiated T lymphocytes, NK cells, and some epithelial cells and endothelial cells. It is not expressed on B cells, monocytes, or granulocytes.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: Mouse N-terminus of CXCR3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone CXCR3-173 does not work for Western blotting.7 For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 126526) with endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Krug A, et al. 2002. J. Immunol. 169:6079. McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed Barbi J, et al. 2009. FASEB J. 23:3990. PubMed Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed Dürr C, et al. 2010. Cancer Res. 70:10170. (Block) PubMed Uppaluri R, et al. 2008. Transplantation 86:137.\u003cbr\u003e\nProduct Citations: Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed Cortés JR, et al. 2022. Cell Rep. 39:110695. PubMed Valencia–Hernandez AM, et al. 2020. Cell Host \u0026amp; Microbe. 27(6):950-962. PubMed Kim CJ, et al. 2018. Immunity. 49:1034. PubMed Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed Schwartz DM et al. 2019. Immunity. 50(1):106-120 . PubMed Mirshahi F, et al. 2022. Cell Rep. 38:110454. PubMed Pein M, et al. 2020. Nat Commun. 11:1494. PubMed Yuan B, et al. 2021. J Immunother Cancer. 9:. PubMed Pais Ferreira D, et al. 2020. Immunity. 53(5):985-1000.e11. PubMed Sebastian M, et al. 2016. J Immunol. 196: 144 - 155. PubMed Guo Y, et al. 2020. Cell Host \u0026amp; Microbe. 28(1):104-116.e4.. PubMed Timilshina M, et al. 2017. PLoS One. 10.1371\/journal.pone.0168942. PubMed Barbi J, et al. 2009. FASEB J. 3.729166667. PubMed Engel I, et al. 2016. Nat Immunol. 10.1038\/ni.3437. PubMed Wang Q, et al. 2013. Genes Dev. 27:1568. PubMed Kimpfler S, et al. 2009. J Immunol. 183:6330. PubMed Li H, et al. 2021. Protein Cell. Online ahead of print. PubMed Meunier I, et al. 2015. PLoS One. 10: 0138055. PubMed Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed Oghumu S, et al. 2013. J Immunol. 190:2229. PubMed McAleer J, et al. 2011. Int Immunol. 1.047916667. PubMed Fend L, et al. 2014. Cancer Immunol Res. 2:1163. PubMed Gardner A, et al. 2022. J Immunother Cancer. 10:. PubMed Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed\u003cbr\u003e\nRRID: AB_1027656 (BioLegend Cat. No. 126505) AB_1027650 (BioLegend Cat. No. 126506)\u003cbr\u003e\nDistribution: T cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors\u003cbr\u003e\nFunction: Essential in T cell recruitment to sites of inflammation\u003cbr\u003e\nCell Type: Dendritic cells, Hematopoietic stem and progenitors, T cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nGene ID: 12766\u003cbr\u003e\nUniProt: View information about CD183 on UniProt.org\u003cbr\u003e\nClone: CXCR3-173\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R )\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862997618857,"sku":"126505","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-126505","provider":"Iright","version":"1.0","type":"link"}