{"product_id":"biolegend-126531","title":"Biolegend, 126531, Brilliant Violet 650™ anti-mouse CD183 (CXCR3) Antibody, 50μg","description":"\u003cp\u003eCD183, also known as CXCR3, is a member of the C-X-C chemokine family, characterized by a pair of cysteine residues separated by a single amino acid. CXCR3 is a 38 kD seven pass transmembrane receptor coupled to G-protein. It mediates Ca 2+ mobilization and chemotaxis in response to C-X-C chemokines, such as IP10 (CXCL10), MIG (CXCL9), I-TAC (CXCL11) and PF4 (CXCL4). CXCR3 is expressed primarily on activiated T lymphocytes, NK cells, and some epithelial cells and endothelial cells. It is not expressed on B cells, monocytes, or granulocytes.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: Mouse N-terminus of CXCR3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660\/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Clone CXCR3-173 does not work for Western blotting.7 For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 126526) with endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Krug A, et al. 2002. J. Immunol. 169:6079. McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed Barbi J, et al. 2009. FASEB J. 23:3990. PubMed Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed Dürr C, et al. 2010. Cancer Res. 70:10170. (Block) PubMed Uppaluri R, et al. 2008. Transplantation 86:137.\u003cbr\u003e\nProduct Citations: Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Clark JT, et al. 2023. Cell Rep. 42:112147. PubMed VanDyke D, et al. 2022. Cell Rep. 41:111478. PubMed Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed Muthuswamy R, et al. 2021. J Immunother Cancer. 9:. PubMed Su W, et al. 2020. Cell Metabolism. 32(6):996-1011.e7. PubMed Marinaik CB, et al. 2020. Cell Rep Med. 1:100095. PubMed Prosser A, et al. 2021. Cell Reports. 35(7):109141. PubMed Picard M, et al. 2020. Cell Death Differ. . PubMed Dobson HE, et al. 2020. Mucosal Immunol. 0.901388889. PubMed Beura LK, et al. 2018. Immunity. 48:327. PubMed Huang H, et al. 2021. Cell. 184(5):1245-1261.e21. PubMed Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed\u003cbr\u003e\nRRID: AB_2563160 (BioLegend Cat. No. 126531)\u003cbr\u003e\nDistribution: T cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors\u003cbr\u003e\nFunction: Essential in T cell recruitment to sites of inflammation\u003cbr\u003e\nCell Type: Dendritic cells, Hematopoietic stem and progenitors, T cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nGene ID: 12766\u003cbr\u003e\nUniProt: View information about CD183 on UniProt.org\u003cbr\u003e\nClone: CXCR3-173\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R )\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862999257257,"sku":"126531","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-126531","provider":"Iright","version":"1.0","type":"link"}