{"product_id":"biolegend-126705","title":"Biolegend, 126705, PE anti-mouse\/human Galectin-3 (Mac-2) Antibody, 50μg","description":"\u003cp\u003eGalectin-3 (galactose-specific lectin 3) is a glycoprotein, also known as Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, and εBP. It is a member of animal β-galactoside-binding protein family that is expressed in cell nucleus, cytoplasm, plasma membrane, or extracellular matrix by variety of tumor cells, monocytes\/macrophages, epithelial cells, Kupffer cells, and dendritic cells. Galectin-3 contains carbohydrate recognition domains and binds to β-galactoside residues bearing glycoproteins, such as as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, and CD66. Galectin-3 is an adhesion molecule and plays an important role in regulation of cell proliferation, differentiation and tumor cell metastasis. Galectin-3 structurely possesses NWGR motif that is conserved in BH-1 domain of BCL-2 family and functions as an anti-apoptotic molecule.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse, Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human Recombinant (partial), amino acids 151-251\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Based on immunogen information, clone Gal397 may recognize African Green Baboon and Cynomolgus. It is recommended to test the reagent for optimal performance for other species. Due to in-house testing, the purified format is not recommended for flow cytometry and intracellular flow cytometry.\u003cbr\u003e\nProduct Citations: Fei F, et al. 2022. Int J Mol Sci. 23:. PubMed Nielsen MA, et al. 2022. Front Immunol. 13:915890. PubMed Zhang X, et al. 2016. J. Clin. Pathol.. 69:1100-1104. PubMed Paz H, et al. 2018. J Exp Clin Cancer Res. 37:67. PubMed Ramos-Medina R, et al. 2011. Immunol. Lett. 134:150-6. PubMed\u003cbr\u003e\nRRID: AB_1134012 (BioLegend Cat. No. 126706)\u003cbr\u003e\nStructure: Human Galetcin-3 has 250 amino acids with a predicted molecular weight of 26 kD.Mouse Galetcin-3 has 264 amino acids with a predicted molecular weight of 27.5 kD.\u003cbr\u003e\nDistribution: Localized in the nucleus, cytoplasm, cell surface, or extracellular matrix. Expressed by tumor cells, monocytes\/macrophages, epithelial cells, Kupffer cells, dendritic cells\u003cbr\u003e\nFunction: Regulate cell proliferation and differentiation, adhesion, anti-apoptosis\u003cbr\u003e\nLigand\/Receptor: Variety ligands, such as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, CD66, etc\u003cbr\u003e\nCell Type: Dendritic cells, Epithelial cells, Macrophages, Monocytes\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Cell Cycle\/DNA Replication, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules\u003cbr\u003e\nAntigen References: Kim HRC, et al. 1999. Cancer Res. 59:4148 Reljic R, et al. 2004. Immunol. Lett. 93:51. Takenaka Y et al. 2004. Mol. and Cell. Biol. 24:4395 Joo GH, et al. 2001. J. Leukoc. Biol. 69:555 Llinas L, et al. 2011. Immunol. Lett. 134:113-21 Cabezón R, et al. 2011. Immunol. Lett. 134:167-73\u003cbr\u003e\nGene ID: 168543958\u003cbr\u003e\nUniProt: View information about Mac-2 on UniProt.org\u003cbr\u003e\nClone: Gal397\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866100453545,"sku":"126705","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-126705","provider":"Iright","version":"1.0","type":"link"}