{"product_id":"biolegend-127015","title":"Biolegend, 127015, APC anti-mouse CD317 (BST2, PDCA-1) Antibody, 25μg","description":"\u003cp\u003eCD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 is a recently identified, IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse plasmacytoid dendritic cells (DCs)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, functional assay2, and depletion3,4. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt;0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Blasius AL, et al. 2006. J. Immunol. 177:3260. Schliemann C, et al. 2010. Blood 115:736. (FA, IF) Rajagopal D, et al. 2010. Blood 115:1949. (Depletion) Moniz RJ, et al. 2010. FEMS Immunol. Med. Microbiol. 58:397. (Depletion) Chen YL, et al. 2013. J Exp Med. 210:2515. PubMed\u003cbr\u003e\nProduct Citations: Wagner C, et al. 2020. Cell Reports. 31(1):107479. PubMed Wang YC, et al. 2023. J Exp Med. 220:. PubMed Kitagawa S, et al. 2022. NPJ Vaccines. 7:115. PubMed Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed Han Y, et al. 2015. J Immunol. 195: 4126 - 4135. PubMed Billi AC, et al. 2019. JCI Insight. 4:8. PubMed Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed Mulas F, et al. 2020. Cell Mol Immunol. . PubMed Sauma D, et al. 2017. Immunol Res. 10.1007\/s12026-017-8936-9. PubMed Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed Hirata SI, et al. 2020. Allergy. 75:1939. PubMed Yang P et al. 2019. Cell Syst. 8(5):427-445 . PubMed Bi E, et al. 2018. J Clin Invest. 128:4821. PubMed Matsuda T, et al. 2022. iScience. 25:105324. PubMed Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed Walker MT, et al. 2021. Front. Allergy. 2:. PubMed Conrad C, et al. 2018. Nat Commun. 9:25. PubMed Chen R, et al. 2021. Front Oncol. 11:743050. PubMed\u003cbr\u003e\nRRID: AB_1967101 (BioLegend Cat. No. 127015) AB_1967127 (BioLegend Cat. No. 127016)\u003cbr\u003e\nStructure: Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.\u003cbr\u003e\nDistribution: Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.\u003cbr\u003e\nFunction: Recently identified antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.\u003cbr\u003e\nCell Type: Dendritic cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Douglas JL. et al. 2009. J Virol. 83(16):7931 2. Cao W et al. 2009. J. Exp. Med. 206(7):1603 3. Neil SJ. et al. 2008. Nature 451:425\u003cbr\u003e\nGene ID: 69550\u003cbr\u003e\nUniProt: View information about CD317 on UniProt.org\u003cbr\u003e\nClone: 927\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862499676329,"sku":"127015","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-127015","provider":"Iright","version":"1.0","type":"link"}