{"product_id":"biolegend-127021","title":"Biolegend, 127021, PerCP\/Cyanine5.5 anti-mouse CD317 (BST2, PDCA-1) Antibody, 25μg","description":"\u003cp\u003eCD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 is a recently identified, IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse plasmacytoid dendritic cells (DCs)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, functional assay2, and depletion3,4. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt;0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Blasius AL, et al. 2006. J. Immunol. 177:3260. Schliemann C, et al. 2010. Blood 115:736. (FA, IF) Rajagopal D, et al. 2010. Blood 115:1949. (Depletion) Moniz RJ, et al. 2010. FEMS Immunol. Med. Microbiol. 58:397. (Depletion) Chen YL, et al. 2013. J Exp Med. 210:2515. PubMed\u003cbr\u003e\nProduct Citations: Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed Liu S, et al. 2020. Cell Host \u0026amp; Microbe. 26(6):779-794.e8.. PubMed Ghorbani S, et al. 2022. Nat Commun. 13:2445. PubMed Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed\u003cbr\u003e\nRRID: AB_2566646 (BioLegend Cat. No. 127021) AB_2566647 (BioLegend Cat. No. 127022)\u003cbr\u003e\nStructure: Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.\u003cbr\u003e\nDistribution: Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.\u003cbr\u003e\nFunction: Recently identified antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.\u003cbr\u003e\nCell Type: Dendritic cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Douglas JL. et al. 2009. J Virol. 83(16):7931 2. Cao W et al. 2009. J. Exp. Med. 206(7):1603 3. Neil SJ. et al. 2008. Nature 451:425\u003cbr\u003e\nGene ID: 69550\u003cbr\u003e\nUniProt: View information about CD317 on UniProt.org\u003cbr\u003e\nClone: 927\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862500233385,"sku":"127021","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-127021","provider":"Iright","version":"1.0","type":"link"}