{"product_id":"biolegend-127209","title":"Biolegend, 127209, APC anti-mouse CD73 Antibody, 25μg","description":"\u003cp\u003eCD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b + myeloid cells. In spleen, it is largely expressed on T cells.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CHO cells transfected with mouse CD73\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)\u003cbr\u003e\nProduct Citations: Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed MacLean AJ, et al. 2022. Immunity. 55:718. PubMed Saxena V, et al. 2022. Cell Rep. 39:110727. PubMed Moor AE et al. 2018. Cell. 175(4):1156-1167 . PubMed Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed Sarin S et al. 2018. Neuron. 98(1):109-126 . PubMed Kleine Holthaus SM, et al. 2018. Mol Ther. 26:1343. PubMed Hou M, et al. 2020. Stem Cell Res Ther. 11:55. PubMed Rebekka K Schneider et al. 2017. Cell stem cell. 20(6):785-800 . PubMed Becher J, et al. 2018. Dev Cell. 47:592. PubMed Ortín-Martínez A, et al. 2021. EMBO J. 40:e107264. PubMed Shen YC, et al. 2021. Nat Commun. 12:3876. PubMed Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed Ben-Moshe S, et al. 2019. Nat Metab. 1:899. PubMed Shevach H 2016. J Immunol. 196: 1517 - 1528. PubMed Vieira JMF, et al. 2022. PLoS One. 17:e0272962. PubMed\u003cbr\u003e\nRRID: AB_11219400 (BioLegend Cat. No. 127209) AB_11218786 (BioLegend Cat. No. 127210)\u003cbr\u003e\nStructure: 69 kD GPI-anchored surface protein\u003cbr\u003e\nDistribution: Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73\u003cbr\u003e\nFunction: A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates\u003cbr\u003e\nLigand\/Receptor: Nucleoside 5' monophosphate\u003cbr\u003e\nCell Type: B cells, Mesenchymal Stem Cells, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981. 2. Resta R, et al. 1998. Immunol. Rev. 161:95.\u003cbr\u003e\nRegulation: Expression is regulated by the development and maturation of leukocytes\u003cbr\u003e\nGene ID: 23959\u003cbr\u003e\nUniProt: View information about CD73 on UniProt.org\u003cbr\u003e\nClone: TY\/11.8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ecto-5'-nucleotidase\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864061333673,"sku":"127209","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-127209","provider":"Iright","version":"1.0","type":"link"}