{"product_id":"biolegend-127407","title":"Biolegend, 127407, PE anti-mouse Podoplanin Antibody, 50μg","description":"\u003cp\u003eThe mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Syrian Hamster\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Farr A, et al. 1992. J. Histochem. Cytochem. 40:651. Farr AG, et al. 1992. J. Exp. Med. 176:1477. Bekiaris V, et al. 2008. J. Immunol. 180:6768. Algars A, et al. 2011. Blood 117:4387. PubMed Reis VO, et al. 2012. Immunobiology. 217:831. PubMed Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC) Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Akasaka H, et al. 2023. JCI Insight. 8:. PubMed Du Y, et al. 2022. FASEB J. 36:e22248. PubMed Krausgruber T, et al. 2020. Nature. 583:296. PubMed Zinselmeyer B, et al. 2013. J Exp Med. 210:757. PubMed Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed Novkovic M, et al. 2020. Cell Reports. 30(3):893-904.e6.. PubMed Luo R, et al. 2021. Theranostics. 11:117. PubMed Chauhan A, et al. 2020. Nat Commun. 11:1939. PubMed Mkrtichyan M, et al. 2012. J Immunol. 189:2450. PubMed Hara T, et al. 2012. J Immunol. 189:1577. PubMed Liu M, et al. 2020. Nature. 587:115. PubMed Emgård J, et al. 2018. Immunity. 143:419. PubMed Bresson L, et al. 2018. Development. 145. PubMed Sun Q, et al. 2015. Bone Res. 3: 15026. PubMed Sullivan B, et al. 2015. PLoS Pathog. 11:1004588. PubMed Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed Baasch S, et al. 2021. Cell. . PubMed Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed Onder L et al. 2017. Immunity. 47(1):80-92 . PubMed Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed Progatzky F, et al. 2021. Nature. 599:125. PubMed Pezoldt J, et al. 2022. Nat Commun. 13:7227. PubMed Chauveau A, et al. 2020. Immunity. 52:794. PubMed Peranzoni E, et al. 2018. Proc Natl Acad Sci U S A. 115:E4041. PubMed Tang C, et al. 2021. Theranostics. 11:9791. PubMed Clement CC, et al. 2021. Nat Commun. 12:4447. PubMed Ito K, et al. 2014. Proc Natl Acad Sci U S A. 111:3080. PubMed Karpus ON, et al. 2019. Cell Rep. 26:3698. PubMed Francipane MG, et al. 2020. Am J Pathol. 190:252. PubMed Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed Kataru RP, et al. 2022. Front Aging. 3:864860. PubMed Mishra A, et al. 2016. J Immunol. 197: 2828 - 2837. PubMed Xu C, et al. 2018. Nat Commun. 9:2449. PubMed Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed Kataru RP, et al. 2019. Cancer Immunol Res. 7:1345. PubMed Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed Goldberg AR, et al. 2022. Front Physiol. 13:1021038. PubMed Bieniasz-Krzywiec P, et al. 2021. STAR Protoc. 2:100481. PubMed Othy S, et al. 2013. J Immunol. 190:4535. PubMed Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed\u003cbr\u003e\nRRID: AB_2161929 (BioLegend Cat. No. 127407) AB_2161928 (BioLegend Cat. No. 127408)\u003cbr\u003e\nStructure: 43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.\u003cbr\u003e\nDistribution: Expressed on epithelial and mesothelial cells.\u003cbr\u003e\nCell Type: Epithelial cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nAntigen References: 1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651. 2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.\u003cbr\u003e\nGene ID: 14726\u003cbr\u003e\nUniProt: View information about Podoplanin on UniProt.org\u003cbr\u003e\nClone: 8.1.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T1a, gp38, stromal cell marker\u003cbr\u003e\nIsotype: Syrian Hamster IgG\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862490763433,"sku":"127407","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-127407","provider":"Iright","version":"1.0","type":"link"}