{"product_id":"biolegend-127627","title":"Biolegend, 127627, Brilliant Violet 421™ anti-mouse Ly-6G Antibody, 125microl","description":"\u003cp\u003eLymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.\u003cbr\u003e\n125microl\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Ly-6G transfected EL-4J cell line.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: µg sizes: 0.2 mg\/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality testedIHC-F - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416. Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC) Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC) Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC) Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC) Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC) Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC) Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete) Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete) Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete) Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed Ma C, et al. 2012. J. Leukoc. Biol. 92:1199. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Yang J, et al. 2022. Cancer Res. 82:4247. PubMed Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed Banka AL, et al. 2023. Nat Commun. 14:2462. PubMed Distel JS, et al. 2023. PLoS Pathog. 19:e1011173. PubMed Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed Fang C, et al. 2022. J Pathol. 256:310. PubMed Wang W, et al. 2022. Dev Cell. 57:228. PubMed Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed Wang W, et al. 2022. Cell Rep. 41:111582. PubMed Glaubitz J, et al. 2023. Gut. :. PubMed Ren ZN, et al. 2022. Acta Pharmacol Sin. 43:1264. PubMed Wilden A, et al. 2022. Front Immunol. 13:991295. PubMed Jong RM, et al. 2022. J Immunol. 208:407. PubMed Radovanovic I, et al. 2014. J Immunol. 193:1290. PubMed Minutti CM, et al. 2019. Immunity. 50:645. PubMed Mansouri N, et al. 2019. JCI Insight. 4:e128060. PubMed Wang L, et al. 2020. Cells. 0.458333333. PubMed Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed Khamissi FZ, et al. 2022. Sci Adv. 8:eabm5900. PubMed Han H, et al. 2021. Front Pharmacol. 12:726586. PubMed Park JG, et al. 2021. iScience. 24(9):102941. PubMed Gu H, et al. 2021. Immun Inflamm Dis. 9:1686. PubMed Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed Pan H, et al. 2020. Mol Psychiatry. . PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Wang LT, et al. 2021. STAR Protocols. 2(1):100337. PubMed Angelidis I, et al. 2019. Nat Commun. 10:963. PubMed Zhang X, et al. 2022. Pharmaceutics. 14:. PubMed Lee RD, et al. 2021. Nat Commun. 12:6843. PubMed Ma M, et al. 2021. Front Immunol. 12:718779. PubMed Green DP, et al. 2019. Neuron. 101:412. PubMed Peres-Emidio EC, et al. 2022. Front Cell Infect Microbiol. 12:811474. PubMed Dane EL, et al. 2022. Nat Mater. 21:710. PubMed Lissner MM, et al. 2020. Elife. 9:00. PubMed Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed Barman PK, et al. 2019. J Immunol. 202:2720. PubMed Ajina R, et al. 2021. Cancer Immunol Res. 9:386. PubMed Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed Pereira JA, et al. 2020. Hum Mol Genet. 29:1253. PubMed Baudoux T, et al. 2018. Sci Rep. 4.0375. PubMed Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed Ghandhi SA, et al. 2022. Sci Rep. 12:14124. PubMed Yu Y, et al. 2022. Nat Commun. 13:6857. PubMed Hassan AO, et al. 2021. Cell Reports. 36(4):109452. PubMed Köhler D, et al. 2020. Nat Commun. 11:1315. PubMed Vicencio JM, et al. 2022. Cell Death Dis. 13:274. PubMed Liu T, et al. 2022. Front Immunol. 13:901349. PubMed Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed Zeng Z, et al. 2022. Oncogene. :. PubMed Li YN, et al. 2022. Nat Commun. 13:4074. PubMed Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed Liyanage S, et al. 2016. Exp Eye Res. 151:160-70. PubMed Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed Pundir P, et al. 2020. Cell Host \u0026amp; Microbe. 26(1):114-122. PubMed Andrade ACDSP, et al. 2021. J Virol. 95:e0127621. PubMed Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed Zeng Q, et al. 2022. iScience. 25:105151. PubMed Niderla-Bielinska J, et al. 2021. Int J Mol Sci. :22. PubMed Wang LT, et al. 2020. Cell Rep. 32:108188. PubMed Kullberg M, et al. 2015. Nanomedicine. 11: 1355-1363. PubMed Muri J, et al. 2020. eLife. 9:e53627.. PubMed Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed Olson B, et al. 2021. Brain Behav Immun. 97:102. PubMed Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Gardner P, et al. 2017. Sci Rep. 7:40830. PubMed Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed Morein D, et al. 2021. Cells. 10: . PubMed La Salvia S, et al. 2020. Am J Physiol Renal Physiol. 319:F868. PubMed Bertuzzi M, et al. 2014. PLoS Pathog. 10:1004413. PubMed Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed Herbst S, et al. 2015. EMBO Mol Med. 30:240. PubMed Nepal S, et al. 2019. Proc Natl Acad Sci U S A. 116:16513. PubMed Zheng C, et al. 2021. J Extracell Vesicles. 10:e12109. PubMed Li H, et al. 2021. J Clin Invest. 131:. PubMed Xiong S, et al. 2020. J Clin Invest. 130:3684. PubMed Lau A, et al. 2022. Sci Adv. 8:eabm0142. PubMed Kennedy J, et al. 2014. J Exp Med. 211:2519. PubMed\u003cbr\u003e\nRRID: AB_10897944 (BioLegend Cat. No. 127627) AB_2562567 (BioLegend Cat. No. 127628)\u003cbr\u003e\nStructure: A 21-35 kD GPI-anchorded membrane protein\u003cbr\u003e\nDistribution: Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.\u003cbr\u003e\nCell Type: Granulocytes, Macrophages, Monocytes\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nAntigen References: Fleming TJ, et al. 1993. J. Immunol. 151:2399.\u003cbr\u003e\nGene ID: 546644\u003cbr\u003e\nUniProt: View information about Ly-6G on UniProt.org\u003cbr\u003e\nClone: 1A8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lymphocyte antigen 6 complex, locus G\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860838011049,"sku":"127627","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-127627","provider":"Iright","version":"1.0","type":"link"}