{"product_id":"biolegend-127641","title":"Biolegend, 127641, Brilliant Violet 650™ anti-mouse Ly-6G Antibody, 50μg","description":"\u003cp\u003eLymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Ly-6G transfected EL-4J cell line.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660\/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416. Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC) Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC) Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC) Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC) Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC) Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC) Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete) Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete) Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete) Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed Ma C, et al. 2012. J. Leukoc. Biol. 92:1199. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed Zhai M, et al. 2022. Nat Commun. 13:7500. PubMed Choi H, et al. 2023. J Nanobiotechnology. 21:5. PubMed Hu K, et al. 2022. J Extracell Vesicles. 11:e12199. PubMed Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed Borkner L, et al. 2021. Mucosal Immunol. 14:1183. PubMed Kim YS, et al. 2021. Cell Death Dis. 12:307. PubMed Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed Fujii Y, et al. 2022. JBMR Plus. 6:e10562. PubMed Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed Moreau GB, et al. 2020. Am J Trop Med Hyg. 103:1215. PubMed Zong F, et al. 2022. Front Immunol. 13:900755. PubMed Yu S, et al. 2016. Mol Ther. 10.1038\/mt.2016.175. PubMed Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed Wang ECE, et al. 2019. Cell Stem Cell. 24:654. PubMed Garcia LR, et al. 2021. Nat Commun. 12:3364. PubMed DiPiazza A, et al. 2017. Sci Rep. 10.1038\/s41598-017-11313-x. PubMed Fujii T, et al. 2021. Bone Res. 9:4. PubMed Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed Utz SG, et al. 2020. Cell. 181(3):557-573. PubMed Sung PS, et al. 2022. JCI Insight. :. PubMed Yun H, et al. 2020. Immunity. 53(5):1050-1062.e5. PubMed Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed Donlan AN, et al. 2021. JCI Insight. 6: . PubMed Poillet–Perez L, et al. 2018. Nature. 563:569. PubMed Tao Z, et al. 2022. Cells. 11:. PubMed Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed\u003cbr\u003e\nRRID: AB_2565881 (BioLegend Cat. No. 127641)\u003cbr\u003e\nStructure: A 21-35 kD GPI-anchorded membrane protein\u003cbr\u003e\nDistribution: Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.\u003cbr\u003e\nCell Type: Granulocytes, Macrophages, Monocytes\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nAntigen References: Fleming TJ, et al. 1993. J. Immunol. 151:2399.\u003cbr\u003e\nGene ID: 546644\u003cbr\u003e\nUniProt: View information about Ly-6G on UniProt.org\u003cbr\u003e\nClone: 1A8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lymphocyte antigen 6 complex, locus G\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860838240425,"sku":"127641","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-127641","provider":"Iright","version":"1.0","type":"link"}