{"product_id":"biolegend-127825","title":"Biolegend, 127825, Brilliant Violet 421™ anti-mouse TCR Vα2 Antibody, 50μg","description":"\u003cp\u003eThe TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ\/δ).  TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Soluble TCR from mouse CTL clone KB5-C20\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: The B20.1 antibody recognizes most members of the Va2 TCR subfamily in mice having the a, b, and c haplotypes.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Pircher H, et al. 1992. Eur. J. Immunol.. 22:399. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed Rao RR, et al. 2012. Immunity. 36:374. PubMed.\u003cbr\u003e\nProduct Citations: Nyberg WA, et al. 2023. Cell. 186:446. PubMed Pasciuto E, et al. 2020. Cell. 182:625. PubMed\u003cbr\u003e\nRRID: AB_2814020 (BioLegend Cat. No. 127825)\u003cbr\u003e\nDistribution: On a subset of T cells\u003cbr\u003e\nFunction: In combination of V β, Vα recognizes MHC\/peptide complex on the antigen-presenting cells.\u003cbr\u003e\nInteraction: Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.\u003cbr\u003e\nLigand\/Receptor: TCR recognizes MHC\/peptide complex\u003cbr\u003e\nCell Type: T cells\u003cbr\u003e\nBiology Area: Adaptive Immunity, Immunology\u003cbr\u003e\nMolecular Family: TCRs\u003cbr\u003e\nAntigen References: 1. Kubo RT, et al. 1989. J. Immunol.. 142:2736. 2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399.\u003cbr\u003e\nGene ID: 21473\u003cbr\u003e\nUniProt: View information about TCR Valpha2 on UniProt.org\u003cbr\u003e\nClone: B20.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell receptor Vα 2\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862549287081,"sku":"127825","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-127825","provider":"Iright","version":"1.0","type":"link"}