{"product_id":"biolegend-128705","title":"Biolegend, 128705, FITC anti-mouse CD199 (CCR9) Antibody, 50μg","description":"\u003cp\u003eCCR9 is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in gut-associated immune response. Mouse CCR9 is highly expressed on different stages of thymocytes and upregulated on CD4+CD8+ cells. It is expressed to a much lower level on spleen and lymph node cells. Expression of CCR9 on γ\/δ T cells in the intraepithelial and small intestine has been reported. The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nProduct Citations: Prior JT, et al. 2023. NPJ Vaccines. 8:80. PubMed Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Sasaki T, et al. 2020. Cell Reports. 28(1):202-217.e7.. PubMed Goubet AG, et al. 2021. Cell Death Differ. 28:2276. PubMed\u003cbr\u003e\nRRID: AB_1186166 (BioLegend Cat. No. 128705) AB_1186167 (BioLegend Cat. No. 128706)\u003cbr\u003e\nStructure: A membrane of G-protein coupled receptors and contains seven transmembrain domains. Mouse CCR9 has above 80% amino acid sequence homology with human counterpart.\u003cbr\u003e\nDistribution: CD4+CD8+ thymocytes, γ\/δ T cells\u003cbr\u003e\nInteraction: CCL25\u003cbr\u003e\nLigand\/Receptor: CCL25\u003cbr\u003e\nCell Type: T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: Zaballos A, et al. 1999. J. Immunol. 162:5671. Wurbel MA, et al. 2007. J. Immunol. 178:7598. Wurbel MA, et al. 2006. Eur. J. Immunol. 36:73.\u003cbr\u003e\nGene ID: 12769\u003cbr\u003e\nUniProt: View information about CD199 on UniProt.org\u003cbr\u003e\nClone: CW-1.2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CCR9, GPR-9-6,GPR28, CDw199\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862995849385,"sku":"128705","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-128705","provider":"Iright","version":"1.0","type":"link"}