{"product_id":"biolegend-128822","title":"Biolegend, 128822, Purified anti-mouse CD226 (DNAM-1) Antibody, 500μg","description":"\u003cp\u003eCD226 (DNAM-1) is constitutively expressed on native CD8 + cells and on CD4 + T cells, macrophages and NK cells.This antibody (10E5) was reported to bind about 40% of inactivated CD4 + cells and binds only to differentiated Th1 cells, but not to Th2 or Th0 cells.It is also reported to suppress antigen-specific T cell expansion and EAE (experimental allergic encephalitis) mediated by Th1 cells.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Th1 polarized T cell clones\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality testedIHC-F - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue, a concentration range of 5.0 - 10.0 µg\/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Dardalhon V, et al. 2005. J. Immunol. 175:1558 Huang Z, et al. 2011. J Immunol. 186:3421. PubMed\u003cbr\u003e\nProduct Citations: Goh W, et al. 2020. Cell Rep. 33:108285. PubMed Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed\u003cbr\u003e\nRRID: AB_2728146 (BioLegend Cat. No. 128822)\u003cbr\u003e\nStructure: 65 kd glycoprotein of a member of Ig-superfamily containing 2 immunologlobulin-like domains.\u003cbr\u003e\nDistribution: Expressed on majority of CD4+ and CD8+ T cells, NK cells, monocytes\/macrophages and a subset of B cells and thymocytes. Reported to be specifically expressed on the surface of Th1 cells, but downregulated in Th2 cells.\u003cbr\u003e\nFunction: Mediates cellular adhesion, involved in LFA-1 costimulatory signal for T cell differentiation and proliferation\u003cbr\u003e\nLigand\/Receptor: CD112 and CD155\u003cbr\u003e\nCell Type: B cells, Macrophages, Monocytes, NK cells, T cells, Th1\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1.Tahara Hanaoka S, et al. 2006. Blood 107:1491 2. Shibuya K, et al. 2003. J. Exp. Med. 198:1829\u003cbr\u003e\nGene ID: 225825\u003cbr\u003e\nUniProt: View information about CD226 on UniProt.org\u003cbr\u003e\nClone: 10E5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: DNAM-1, PTA1 (platelet and T cell activation antigen 1), TLISA1, LFA-1 associated Molecule PTA-1\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860781387945,"sku":"128822","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-128822","provider":"Iright","version":"1.0","type":"link"}