{"product_id":"biolegend-129612","title":"Biolegend, 129612, APC anti-mouse Siglec H Antibody, 100μg","description":"\u003cp\u003eThe Siglec-H is a member of CD33-related Siglec family which mostly expressed in the innate immune system. Siglec-H is reported to be specifically expressed on plasmacytoid dendritic cells. Siglec-H depends on adaptor protein DAP 12 (DNAX-activating protein of 12 kD) for surface expression and signal transduction. Cross-linking with this antibody can selectively inhibit interferon-alpha production by IPCs following TLR9 ligation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Siglec-H-Fc recombinant fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Blasius AL, et al. 2006. J. Immunol. 177:3260.\u003cbr\u003e\nProduct Citations: Lutz MB, et al. 2022. Eur J Immunol. . PubMed Takimoto Y, et al. 2023. iScience. 26:106220. PubMed Rocca Y, et al. 2023. Methods Mol Biol. 2618:187. PubMed Zengel J, et al. 2023. Nat Methods. . PubMed Fontana MF, et al. 2023. Elife. 12: . PubMed Feng J, et al. 2022. Immunity. 55:405. PubMed Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed Weigert A, et al. 2022. Nat Commun. 13:6078. PubMed Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed Kimura T, et al. 2020. Commun Biol. 0.527777778. PubMed Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed Mahmoudi S, et al. 2019. Nature. 574:553. PubMed\u003cbr\u003e\nRRID: AB_10643574 (BioLegend Cat. No. 129611) AB_10641134 (BioLegend Cat. No. 129612)\u003cbr\u003e\nStructure: Sialic acid-binding immunoglobulin-like lectins\u003cbr\u003e\nDistribution: Siglec-H is expressed exclusively on natural interferon-producing cells (IPCs), also called plasmacytoid dendritic cells in spleen, blood and lymph nodes. The IPCs are characterized by a complex phenotype (CD11c+, Ly-6C+, B220+, CD11b-, CD8+\/-).\u003cbr\u003e\nFunction: Siglec-H associates with adaptor protein DAP12 for cell signaling, which reduces type 1 IFN response of IPC to CpG.\u003cbr\u003e\nLigand\/Receptor: Sialic acid\u003cbr\u003e\nCell Type: Dendritic cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules, Siglec Molecules\u003cbr\u003e\nAntigen References: 1. Blasius AL, et al. 2006. Blood 107:2474. 2. Zhang J, et al. 2006. Blood 107:3600. 3. Blasius AL, et al. 2007. J. Exp. Med. 204:2561.\u003cbr\u003e\nGene ID: 233274\u003cbr\u003e\nUniProt: View information about Siglec H on UniProt.org\u003cbr\u003e\nClone: 551\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Sialic acid-binding immunoglobulin-like lectin, SiglecH\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862443184297,"sku":"129612","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-129612","provider":"Iright","version":"1.0","type":"link"}