{"product_id":"biolegend-135012","title":"Biolegend, 135012, APC anti-mouse CD127 (IL-7Rα) Antibody, 100μg","description":"\u003cp\u003eCD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4\/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: IL-7Ra-IgG1 fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Clone A7R34 is able to completely block the binding of clone S18006K to IL-7Rα. It does not block SB\/199.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. Hashi H, et al. 2001. J. Immunol. 166:3702. Taylor R, et al. 2007. J. Immunol. 178:5659. Mazzon C, et al. 2011. Blood. 118:2733. PubMed Jin J, et al. 2011. J. Immunol. doi:10.4049\/jimmunol.1001238. PubMed\u003cbr\u003e\nProduct Citations: Wang X, et al. 2023. Nat Commun. 14:3440. PubMed Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Ali LR, et al. 2023. J Exp Med. 220: . PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed Pfenninger P, et al. 2022. Front Immunol. 13:777113. PubMed Zhao H, et al. 2021. Int J Nanomedicine. 16:4913. PubMed Nakajima T, et al. 2021. J Exp Med. 218:. PubMed Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed Zaro BW, et al. 2020. eLife. 9:00. PubMed Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed Xu X, et al. 2018. J Dermatol Sci. 91:134. PubMed Ding P, et al. 2022. Bone Res. 10:42. PubMed Hu Z, et al. 2020. PLoS One. 15:e0228339. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed Bajaña S, et al. 2022. iScience. 25:103732. PubMed Panagi M, et al. 2020. Theranostics. 10:1910. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Fujimoto M, et al. 2022. Nat Commun. 13:5408. PubMed Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed Ding Z, et al. 2017. Sci Rep. 10.1038\/s41598-017-12488-z. PubMed\u003cbr\u003e\nRRID: AB_1937217 (BioLegend Cat. No. 135011) AB_1937216 (BioLegend Cat. No. 135012)\u003cbr\u003e\nStructure: Type I transmembrane glycoprotein, associate with CD132, 60-90 kD\u003cbr\u003e\nDistribution: Immature B cells through early pre-B stage, thymocytes (except CD4\/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells\u003cbr\u003e\nFunction: T cell and immature B cell proliferation and development\u003cbr\u003e\nLigand\/Receptor: IL-7\u003cbr\u003e\nCell Type: B cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246. 3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.\u003cbr\u003e\nGene ID: 16197\u003cbr\u003e\nUniProt: View information about CD127 on UniProt.org\u003cbr\u003e\nClone: A7R34\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL-7 receptor α chain, IL-7Rα\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862524252329,"sku":"135012","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-135012","provider":"Iright","version":"1.0","type":"link"}