{"product_id":"biolegend-135106","title":"Biolegend, 135106, PE anti-mouse CD117 (c-kit) Antibody, 200μg","description":"\u003cp\u003eCD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and\/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Murine IL-3 dependent mast cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Injection of ACK2 in mice of NOD genetic background is not recommended as mice develop immediate anaphylaxis, resulting in animal death. Treatment is possible with co-injection of Benadryl.3 ACK2 has been reported to block c-Kit function. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 135103). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 135114) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin \u0026lt;0.01 EU\/µg).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yoshinaga K, et al. 1991. Development 113:689. Broudy VC, et al. 1996. Blood 88:75. Louvet C, et al. 2008. Proc. Natl. Acad. Sci. USA 105:18895.\u003cbr\u003e\nProduct Citations: Sandrock I, et al. 2015. PLoS One. 10: 0145010. PubMed Bennion BG, et al. 2020. Cell Reports. 31(11):107771. PubMed Huang WC, et al. 2019. Int J Hyperthermia. 36:1255. PubMed\u003cbr\u003e\nRRID: AB_1877216 (BioLegend Cat. No. 135105) AB_1877215 (BioLegend Cat. No. 135106)\u003cbr\u003e\nStructure: Ig superfamily, 145 kD\u003cbr\u003e\nDistribution: Hematopoietic stem cells, AML, mast cells, oocytes, interstitial theca cells, leydig cells\u003cbr\u003e\nFunction: Growth factor receptor, tyrosine kinase\u003cbr\u003e\nLigand\/Receptor: Stem Cell Factor (SCF)\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Galli SJ. et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P et al. 1986. Nature 320:415. 5. Witte ON. et al. 1990. Cell 63:5.\u003cbr\u003e\nGene ID: 16590\u003cbr\u003e\nUniProt: View information about CD117 on UniProt.org\u003cbr\u003e\nClone: ACK2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: c-KIT, Stem Cell Factor Receptor (SCFR)\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862529134761,"sku":"135106","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-135106","provider":"Iright","version":"1.0","type":"link"}