{"product_id":"biolegend-135510","title":"Biolegend, 135510, APC anti-mouse CD115 (CSF-1R) Antibody, 100μg","description":"\u003cp\u003eCSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes\/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: blocking of ligand binding1. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1154.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Sudo T, et al. 1995. Oncogene 11:2469. Murayama T, et al. 1999. Circulation 99:1740. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. PubMed Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed Dong L, et al. 2016. Nature. 539:304–308. PubMed\u003cbr\u003e\nProduct Citations: Gupta T, et al. 2023. Front Immunol. 14:1044703. PubMed Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed McNamara NB, et al. 2023. Nature. 613:120. PubMed Tunali G, et al. 2023. J Clin Invest. :. PubMed Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed Andrés-Blasco I, et al. 2015. J Endocrinol. 227: 179 - 191. PubMed Hu S, et al. 2018. Front Immunol. 9:01. PubMed Hickey A, et al. 2021. Front Microbiol. 12:653587. PubMed Accarias S, et al. 2020. J Cell Sci. 133:. PubMed Mason HD, et al. 2021. JCI Insight. 6:e149229. PubMed Kobori T, et al. 2018. Front Immunol. 9:334. PubMed Fujii Y, et al. 2022. JBMR Plus. 6:e10562. PubMed Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed Almutairi F, et al. 2021. Front Immunol. 12:772288. PubMed Hong H, et al. 2013. Biochem Biophys Res Commun. 440:545. PubMed Svahn S, et al. 2015. Infect Immun . 83:514. PubMed Izquierdo-Useros N, et al. 2014. J Immunol . 192:4852-4858. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Pedragosa J, et al. 2020. J Cereb Blood Flow Metab. 40:S98. PubMed Shami A, et al. 2015. Sci Rep. 5: 13904. PubMed Saulnier N, et al. 2013. PLoS One. 7:30788. PubMed Galvani S, et al. 2015. Sci Signal. 8: ra79. PubMed Wang J, et al. 2021. Nat Commun. 12:6198. PubMed Herrero–Cervera A, et al. 2019. Diabetologia. 62:2143. PubMed Sauter K, et al. 2014. J Leukoc Biol. 96:265. PubMed Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed Svahn S, et al. 2016. Infect Immun . 84: 1205-1213. PubMed Wang X, et al. 2016. J Immunol. 197(12):4750-4761. PubMed Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed Ledo JH, et al. 2020. PLoS One. e0237773:15. PubMed Huo M, et al. 2017. FASEB J. 10.1096\/fj.201601030R. PubMed Kitamura K, et al. 2019. Int J Cardiol Heart Vasc. 23:100344. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Baratin M, et al. 2017. Immunity. 47:349. PubMed Reed-Geaghan EG, et al. 2020. J Exp Med. 217:00:00. PubMed Asada S, et al. 2018. Nat Commun. 9:2733. PubMed Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed Viaud M, et al. 2020. Cell Reports. 30(10):3397-3410. PubMed Tähtinen S, et al. 2022. Nat Immunol. 23:532. PubMed Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed Zhang B, et al. 2021. Front Neurol. 607370:12. PubMed Ledo JH, et al. 2020. Mol Psychiatry. . PubMed Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed Cleary MM, et al. 2021. Mol Cancer Ther. 20:906. PubMed\u003cbr\u003e\nRRID: AB_2085222 (BioLegend Cat. No. 135509) AB_2085221 (BioLegend Cat. No. 135510)\u003cbr\u003e\nStructure: Ig superfamily, 145 kD\u003cbr\u003e\nDistribution: Monocytes\/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts\u003cbr\u003e\nFunction: Growth factor receptor, tyrosine kinase\u003cbr\u003e\nLigand\/Receptor: Macrophage colony stimulating factor (M-CSF), IL-34\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages, Monocytes, Osteoclasts\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: Sudo T, et al. 1995 Oncogene 11:2469. Murayama T, et al. 1999 Circulation 99:1740. Goswami S, et al. 2005 Cancer Res. 65:5278. Yu W, et al. 2008 J. Leuko. Biol. 84:852.\u003cbr\u003e\nGene ID: 12978\u003cbr\u003e\nUniProt: View information about CD115 on UniProt.org\u003cbr\u003e\nClone: AFS98\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CSF-1R, M-CSFR, c-fms\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: Why do I have a weak CD115 staining?\u003cbr\u003e\nA: It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.  Pre-treatment with EDTA and low temperatures (2 - 8°C) helps in maintaining surface expression of CD115. In addition, brief fixation of the cells with Fixation Buffer (Cat. No. 420801) for 10 minutes will block CD115 internalization.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862535622825,"sku":"135510","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-135510","provider":"Iright","version":"1.0","type":"link"}