{"product_id":"biolegend-137013","title":"Biolegend, 137013, PE anti-mouse CD68 Antibody, 25μg","description":"\u003cp\u003eMouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Purified Con A receptor glycoproteins from the P815 cell line\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality testedFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3, and spatial biology (IBEX)9,10.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB) Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC) Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC) Kayama H, et al. 2012. PNAS. 109:5010. PubMed Park S, et al. 2013. Biomaterials. 34:598. PubMed Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Zhao C, et al. 2013. Int J Pharm. 450:11. PubMed Miho Sato‐Hashimoto et al. 2019. eLife. 8 pii: e42025. PubMed Dona MSI, et al. 2023. Basic Res Cardiol. 118:11. PubMed Deng R, et al. 2022. Adv Sci (Weinh). 9:e2103343. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Bhat N, et al. 2022. J Clin Invest. 132:. PubMed Zhang Y, et al. 2013. J Immunol. 900:4725. PubMed Wei SC, et al. 2020. Cancer Discov. . PubMed Guan Z, et al. 2020. Front Cell Neurosci. 0.763888889. PubMed Li Y, et al. 2021. Brain Behav Immun. 91:267. PubMed Cougnoux A, et al. 2018. Hum Mol Genet. 27:2076. PubMed Cummings H, et al. 2012. Proc Natl Acad Sci U S A. 109:1251. PubMed Lv LL, et al. 2018. J Am Soc Nephrol. 29:919. PubMed Litvinchuk A, et al. 2021. Ann Neurol. 89:952. PubMed Litvinchuk A et al. 2018. Neuron. 100(6):1337-1353 . PubMed Grisanti LA, et al. 2019. JCI Insight. 5. PubMed Osinski V, et al. 2020. Theranostics. 10:585. PubMed Li C, et al. 2020. Cancer Immunol Res. 1.030555556. PubMed O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed\u003cbr\u003e\nRRID: AB_10613469 (BioLegend Cat. No. 137013) AB_10612937 (BioLegend Cat. No. 137014)\u003cbr\u003e\nStructure: A member of the lysosomal-associated membrane protein (lamp) family.\u003cbr\u003e\nDistribution: Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.\u003cbr\u003e\nFunction: Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.\u003cbr\u003e\nCell Type: Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules, Innate Immune Signaling\u003cbr\u003e\nAntigen References: 1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833. 2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.\u003cbr\u003e\nGene ID: 12514\u003cbr\u003e\nUniProt: View information about CD68 on UniProt.org\u003cbr\u003e\nClone: FA-11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Macrosialin\u003cbr\u003e\nIsotype: Rat IgG2a\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863994028201,"sku":"137013","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-137013","provider":"Iright","version":"1.0","type":"link"}