{"product_id":"biolegend-138013","title":"Biolegend, 138013, Brilliant Violet 421™ anti-mouse CD144 (VE-cadherin) Antibody, 50μg","description":"\u003cp\u003eCD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo .\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: VE-cadherin-Ig fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM5. Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy staining4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block) Liao F, et al. 2000. Cancer Res. 60:6805. (FC) Crosby CV, et al. 2005. Blood 105:2771. (FC) Liao F, et al. 2002. Cancer Res. 62:2567. (IF) May C, et al. 2005. Blood 105:4337. (epitope)\u003cbr\u003e\nProduct Citations: Komori R, et al. 2022. Int J Mol Sci. 23: . PubMed Krausgruber T, et al. 2020. Nature. 583:296. PubMed Liu X, et al. 2021. Nat Commun. 12:1832. PubMed Tang W, et al. 2021. Front Cell Dev Biol. 9:728057. PubMed Figueiredo CA, et al. 2022. J Neuroinflammation. 19:17. PubMed\u003cbr\u003e\nRRID: AB_2562627 (BioLegend Cat. No. 138013)\u003cbr\u003e\nStructure: A 120 kD member of type II Cadherin family\u003cbr\u003e\nDistribution: Endothelial cells\u003cbr\u003e\nFunction: Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion\u003cbr\u003e\nLigand\/Receptor: CD144\u003cbr\u003e\nCell Type: Endothelial cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821. 2. Hirashima M, et al. 2009. Blood 93:1253. 3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362. 4. Matsumura K, et al. 2003. Blood 101:1367. 5. Hirashima M, et al. 2009. Blood 101:2261. 6. Gotsch U, et al. 1997. J. Cell Sci. 110:583. 7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.\u003cbr\u003e\nGene ID: 12562\u003cbr\u003e\nUniProt: View information about CD144 on UniProt.org\u003cbr\u003e\nClone: BV13\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Vascular endothelial-cadherin\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862928085161,"sku":"138013","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-138013","provider":"Iright","version":"1.0","type":"link"}