{"product_id":"biolegend-138302","title":"Biolegend, 138302, Purified anti-mouse Ly49D Antibody, 500μg","description":"\u003cp\u003eLy49D is a type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.  Ly49D is expressed on subsets of natural killer (NK) cells in C57BL\/6, C3H\/He and SJL mouse strains, but not Balb\/c, DBA\/2, AKR and CBA\/J.  The receptors form homodimers that bind to MHC class I alloantigens.  Unlike inhibitory Ly49 family members, Ly49D lacks a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) but contains a cytoplasmic immunoreceptor tyrosine-based activation motif.  The activating receptor Ly49D delivers stimulatory signals for target cell lysis upon interacting with H2-D d , D r and D sp2 , but not with H2 b or H2 k class I antigens.  Cross-linking of Ly49D activates intracellular kinase activity and calcium mobilization in NK cells.  In 129\/J mice, 4E5 antibody cross-reacts with Ly49O, LY49R and Ly49V.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking2, 3 of CHO cell or Con A-activated lymphoblast killing by NK cells.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Mason LH, et al. 1998. J. Immunol. 160:4148. (IP) Idris A, et al. 1999. Proc. Natl. Acad. Sci. 96:6330. (FC Block) George TC, et al. 1999. J. Immunol. 162:2035. (FC Block) Ortaldo JR, et al. 1999. J. Leukoc. Biol. 66:512. (FC) Ortaldo JR, et al. 1998. J. Immunol. 160:1158. (FC)\u003cbr\u003e\nProduct Citations: Potempa M, et al. 2022. J Immunol. 208:1742. PubMed Goh W, et al. 2020. Cell Rep. 33:108285. PubMed\u003cbr\u003e\nRRID: AB_10574460 (BioLegend Cat. No. 138302)\u003cbr\u003e\nStructure: A type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.\u003cbr\u003e\nDistribution: Subsets of natural killer (NK) cells in C57BL\/6, C3H\/He and SJL, but not Balb\/c, DBA\/2, AKR and CBA\/J.\u003cbr\u003e\nFunction: Plays an important role in activating natural killer (NK) cell cytolytic activity.\u003cbr\u003e\nLigand\/Receptor: MHC class I alloantigens\u003cbr\u003e\nCell Type: NK cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Yokoyama WM and Seaman WE. 1993. Annu. Rev. Immunol. 11:613. 2. Takei F, et al. 1997. Immunol. Rev. 155:67. 3. Hanke T, et al. 1999. Immunity 11:67. 4. Mason LH, et al. 1996. J. Exp. Med. 184:2119.\u003cbr\u003e\nGene ID: 16635\u003cbr\u003e\nUniProt: View information about Ly49D on UniProt.org\u003cbr\u003e\nClone: 4E5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ly-49D\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862415462569,"sku":"138302","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-138302","provider":"Iright","version":"1.0","type":"link"}