{"product_id":"biolegend-138409","title":"Biolegend, 138409, FITC anti-mouse\/human KLRG1 (MAFA) Antibody, 25μg","description":"\u003cp\u003eKiller cell lectin-like receptor G1 (KLRG1) is the mouse homolog of the rat mast cell function-associated antigen (MAFA or 2F1-Ag).  KLRG1 is a type II membrane glycoprotein that was first identified on the surface of rat mast cell line RBL-2H3.  It is composed of a homodimer of glycosylated 30-38 kD subunits.  Mouse and human homologs of KLRG1 are expressed by subsets of NK cells and lymphokine-activated killer (LAK) cells but not mast cells.  KLRG1 is also expressed on subsets of CD8 + and CD4 + cells, including CD4 + and CD8 + effector\/memory cells, potent regulatory CD4 + T cells.  KLRG1 may be involved in regulating NK cell homeostasis.  KLRG1 was found to recognize cadherins and thus inhibit immune responses by regulating the effector function and the developmental processes of NK and T cells.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse, Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Syrian Hamster\u003cbr\u003e\nImmunogen: IL-2 activated NK cells from C57BL\/6 mice\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: For successful staining of human cells, brighter fluorophore-antibody conjugates, such as PE, BV421™, or APC, are recommended.Additional reported applications (for relevant formats) include: Western Blotting1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Robbins SH, et al. 2002. J. Immunol. 168:2585. (WB) Robbins SH, et al. 2003. J. Immunol. 170:5876. (FC) McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC)\u003cbr\u003e\nProduct Citations: Zheng X, et al. 2019. PLoS Pathog. 15:e1008036. PubMed Thakker P, et al. 2023. PLoS Pathog. 19:e1011455. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed Wang L, et al. 2019. Cell Rep. 29:1848. PubMed Pokrovskii M, et al. 2019. Immunity. 51:185. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed Devalraju KP, et al. 2021. PLoS One. 16:e0257185. PubMed Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed Zebley CC, et al. 2021. Cell Rep. 37:109796. PubMed Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed Blaum F, et al. 2021. Life (Basel). 11:. PubMed Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed Moguche AO et al. 2017. Cell host \u0026amp; microbe. 21(6):695-706 . PubMed Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Zheng Y, et al. 2022. J Immunol. 208:501. PubMed Fujimoto M, et al. 2022. Nat Commun. 13:5408. PubMed Wagner AK, et al. 2022. iScience. 25:105137. PubMed Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed\u003cbr\u003e\nRRID: AB_10643998 (BioLegend Cat. No. 138409) AB_10643582 (BioLegend Cat. No. 138410)\u003cbr\u003e\nDisclaimer: This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.\u003cbr\u003e\nStructure: KLRG1 is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM, expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kD.\u003cbr\u003e\nDistribution: Subsets of NK cells, lymphokine-activated killer (LAK) cells and subsets of CD8+ and CD4+ cells.\u003cbr\u003e\nFunction: Regulates NK cell proliferation, maturation, and homeostasis. Inhibits immune responses by regulating the effector function and the developmental processes of NK and T cells.\u003cbr\u003e\nLigand\/Receptor: E-cadherin\u003cbr\u003e\nCell Type: NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nAntigen References: 1. Grundemann C, et al. 2006. J. Immunol. 176:1311. 2. Blaser C, et al. 1998. J. Immunol. 161:6451. 3. Huntington ND, et al. 2007. J. Immunol. 178:4764. 4. Voehringer D, et al. 2001. J. Immunol. 167:4834.\u003cbr\u003e\nGene ID: 50928\u003cbr\u003e\nUniProt: View information about KLRG1 on UniProt.org\u003cbr\u003e\nClone: 2F1\/KLRG1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MAFA, 2F1-Ag\u003cbr\u003e\nIsotype: Syrian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862322565289,"sku":"138409","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-138409","provider":"Iright","version":"1.0","type":"link"}