{"product_id":"biolegend-139319","title":"Biolegend, 139319, PE\/Dazzle™ 594 anti-mouse CD64 (FcγRI) Antibody, 25μg","description":"\u003cp\u003eCD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes\/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG\/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: BALB\/c mouse FcγRI-human IgG Fc fusion protein.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Dazzle™ 594 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE\/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The X54-5\/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5\/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5\/7.1 is not found to be useful for Western blots1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tan PS, et al. 2003. J. Immunol. 170:2549. (IP) Ingersoll MA, et al. 2010. Blood 115:e10. (FC) Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed Sepe JJ, et al. 2022. JACC Basic Transl Sci. 7:915. PubMed Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed Marangoni F, et al. 2021. Cell. . PubMed Lu S, et al. 2020. JCI Insight. 5:00. PubMed Samarchith P Kurup et al. 2019. Cell host \u0026amp; microbe. 25(4):565-577 . PubMed Forbester JL, et al. 2020. J Virol. 94:. PubMed Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed\u003cbr\u003e\nRRID: AB_2566558 (BioLegend Cat. No. 139319) AB_2566559 (BioLegend Cat. No. 139320)\u003cbr\u003e\nStructure: Ig superfamily, type I glycoprotein, 72 kD\u003cbr\u003e\nDistribution: Monocytes, macrophages, mast cells, dendritic cells\u003cbr\u003e\nFunction: Phagocytosis, ADCC\u003cbr\u003e\nLigand\/Receptor: IgG\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages, Mast cells, Monocytes\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nGene ID: 14129\u003cbr\u003e\nUniProt: View information about CD64 on UniProt.org\u003cbr\u003e\nClone: X54-5\/7.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FcRI\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864098230441,"sku":"139319","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-139319","provider":"Iright","version":"1.0","type":"link"}