{"product_id":"biolegend-139323","title":"Biolegend, 139323, Brilliant Violet 605™ anti-mouse CD64 (FcγRI) Antibody, 50μg","description":"\u003cp\u003eCD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes\/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG\/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: BALB\/c mouse FcγRI-human IgG Fc fusion protein.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610\/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: The X54-5\/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5\/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5\/7.1 is not found to be useful for Western blots1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tan PS, et al. 2003. J. Immunol. 170:2549. (IP) Ingersoll MA, et al. 2010. Blood 115:e10. (FC) Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed Zaman R, et al. 2021. Immunity. :. PubMed Roussel-Queval A, et al. 2023. STAR Protoc. 4:102119. PubMed Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed Howard ZM, et al. 2022. Am J Physiol Cell Physiol. 322:C354. PubMed Bhattacharjee A, et al. 2021. Immunity. 54(8):1745-1757.e7. PubMed Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed Dong L, et al. 2021. Cancer Cell. . PubMed Huggins MA, et al. 2019. Cell Rep. 28:1729. PubMed Chopin M et al. 2018. Immunity. 50(1):77-90 . PubMed Warheit-Niemi HI, et al. 2022. JCI Insight. 7:. PubMed Guo P, et al. 2021. J Immunol. 207:408. PubMed Liu S, et al. 2020. Cell Host \u0026amp; Microbe. 26(6):779-794.e8.. PubMed Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed Unudurthi SD, et al. 2020. Life Sci. 117440:247. PubMed Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed Howard ZM, et al. 2022. JCI Insight. 7:. PubMed Sung PS, et al. 2022. JCI Insight. :. PubMed Molgora M, et al. 2020. Cell. 182:886. PubMed Ryu S, et al. 2021. eLife. 0.416666666666667. PubMed Huang X, et al. 2021. Immunity. . PubMed Goc J, et al. 2021. Cell. 184:5015. PubMed Yang J, et al. 2022. Ann Transl Med. 10:1089. PubMed Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed Trivedi S, et al. 2020. Elife. 9:00. PubMed Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed\u003cbr\u003e\nRRID: AB_2629778 (BioLegend Cat. No. 139323)\u003cbr\u003e\nStructure: Ig superfamily, type I glycoprotein, 72 kD\u003cbr\u003e\nDistribution: Monocytes, macrophages, mast cells, dendritic cells\u003cbr\u003e\nFunction: Phagocytosis, ADCC\u003cbr\u003e\nLigand\/Receptor: IgG\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages, Mast cells, Monocytes\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nGene ID: 14129\u003cbr\u003e\nUniProt: View information about CD64 on UniProt.org\u003cbr\u003e\nClone: X54-5\/7.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FcRI\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864098820265,"sku":"139323","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-139323","provider":"Iright","version":"1.0","type":"link"}