{"product_id":"biolegend-140413","title":"Biolegend, 140413, Pacific Blue™ anti-mouse CD8b.2 Antibody, 25μg","description":"\u003cp\u003eCD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse thymus or spleen\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4 of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ledbetter J, et al. 1979. Immunol. Rev. 47:63. Ledbetter J, et al. 1980. J. Exp. Med. 152:280. Vremec D, et al. 2000. J. Immunol. 164:2978. (IF) Lawrence D, et al. 1999. J. Virol. 73:1795. (IHC) Bosselut R, et al. 1999. J. Exp. Med. 190:1517. (IP)\u003cbr\u003e\nProduct Citations: Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed Lercher A, et al. 2019. Immunity. 51:1074. PubMed Nika L, et al. 2019. Vaccines (Basel). 7. PubMed Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed\u003cbr\u003e\nRRID: AB_10641278 (BioLegend Cat. No. 140414)\u003cbr\u003e\nStructure: Ig superfamily, CD8β chain, 32 kD\u003cbr\u003e\nDistribution: Majority of thymocytes, cytotoxic T cell subsets\u003cbr\u003e\nFunction: Co-receptor of TCR\u003cbr\u003e\nLigand\/Receptor: MHC class I\u003cbr\u003e\nCell Type: T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, TCRs\u003cbr\u003e\nAntigen References: 1. Ledbetter J, et al. 1981. J. Exp. Med. 153:1503. 2. Renard V, et al. 1996. J. Exp. Med. 184:2439.\u003cbr\u003e\nGene ID: 12526\u003cbr\u003e\nUniProt: View information about CD8beta.2 on UniProt.org\u003cbr\u003e\nClone: 53-5.8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lyt-3.2, Ly-3.2\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862431191209,"sku":"140413","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-140413","provider":"Iright","version":"1.0","type":"link"}