{"product_id":"biolegend-141613","title":"Biolegend, 141613, APC\/Fire™ 750 anti-mouse H-2K, 25μg","description":"\u003cp\u003eThis antibody has been proven to be very useful in tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo .\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: SIINFEKL pulsed RMA-S cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC\/Fire™ 750 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * APC\/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide. Additional reported applications (for relevant formats) include: immunofluorescence microscopy2,3, immunohistochemical staining of frozen tissue sections3 , and inhibition of T cell response to H-2Kb-SIINFEKL in vitro.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Mareeva T, et al. 2010. J. Immunol. Methods 353:78. Dolan BP, et al. 2010. J. Immunol. 184:1419. (IF) Porgador A, et al. 1997. Immunity 6:715. (IF, IHC) Herve J, et al. 2013. J. Immunol. 190:3163. PubMed.\u003cbr\u003e\nProduct Citations: Tjärnhage E, et al. 2023. J Virol. e0169422:97. PubMed\u003cbr\u003e\nRRID: AB_2750523 (BioLegend Cat. No. 141613) AB_2750524 (BioLegend Cat. No. 141614)\u003cbr\u003e\nStructure: OVA peptide257-264 and H-2Kb complex\u003cbr\u003e\nDistribution: SIINFEKL bond H-2Kb bearing cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78. 2. Mareeva T, et al. 2008. J. Biol. Chem. 283:29053. 3. Deng Y, et al. 1998. J. Immunol. 161:1677.\u003cbr\u003e\nGene ID: 14972\u003cbr\u003e\nUniProt: View information about H-2Kb bound to SIINFEKL on UniProt.org\u003cbr\u003e\nClone: 25-D1.16\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class I molecule Kb bound to the peptide SIINFEKL (Kb-SIINFEKL) H-2Kb, OVA agonist peptide (OVAp), OVA-Kb, OVA257-264 (SIINFEKL)-H-2kb, OVA257-264-H-2kb\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860880150697,"sku":"141613","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-141613","provider":"Iright","version":"1.0","type":"link"}