{"product_id":"biolegend-141805","title":"Biolegend, 141805, PE anti-mouse Allergin-1 Antibody, 25μg","description":"\u003cp\u003eAllergin-1, also known as mast cell Ag-32, is a 60 kD single pass transmembrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain in the extracellular region and one immunoreceptor tyrosine-based inhibitory motif (ITIM)-like domain in the cytoplasmic region. Allergin-1 is expressed on mast cells, dendritic cells, and macrophages as well as at low levels on neutrophils. After tyrosine phosphorylation, allergin-1 recruits PTPN6, PTPN11, and INPP5D, which results in a diminution of IgE-mediated degranulation in mast cells. No ligands have been identified for this molecule.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Transfected cells expressing allergin-1-Flag\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Inhibits FcεRI-mediated degranulation in bone marrow-derived cultured mast cells1. Additional reported applications (for the relevant formats) include: blocking1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hitomi K, et al. 2010. Nat. Immunol. 11:601. (Block)\u003cbr\u003e\nRRID: AB_10900438 (BioLegend Cat. No. 141805)\u003cbr\u003e\nStructure: Single pass transmembrane protein of 60 kD; contains one Ig-like C2-like domain and one ITIM-like domain\u003cbr\u003e\nDistribution: Mast cells, dendritic cells, macrophages, neutrophils\u003cbr\u003e\nFunction: Inhibits IgE-mediated degranulation of mast cells\u003cbr\u003e\nInteraction: PTPN6, PTPN11 and INPP5D\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages, Mast cells, Neutrophils\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Hitomi K, et al. 2010. Nat. Immunol. 11:601. 2. Pirozzi G, et al. 1995. J. Immunol. 155:5811.\u003cbr\u003e\nGene ID: 380732\u003cbr\u003e\nUniProt: View information about Allergin-1 on UniProt.org\u003cbr\u003e\nClone: TX83\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Allergy inhibitory receptor 1, mast cell Ag-32, MCA-32, Milr1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864061137065,"sku":"141805","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-141805","provider":"Iright","version":"1.0","type":"link"}