{"product_id":"biolegend-141904","title":"Biolegend, 141904, PE anti-mouse CD283 (TLR3) Antibody, 100μg","description":"\u003cp\u003eToll-like receptor 3 (TLR3), also known as CD283, is type I transmembrane protein that belongs to the TLR family. It forms a large horseshoe shape that contacts a neighboring horseshoe, forming a dimer of two horseshoes. It is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. TLR3 is expressed selectively on the cytoplasmic membrane and intracellularly in dendritic cells. It is also highly expressed in the placenta, pancreas, heart, liver, lung, and muscle. TLR3 is a pattern recognition receptor that participates in innate immune response to microbial pathogens by recognizing polyinosine-polycytidylic acid (Poly(I:C)) and dsRNA. Ligand binding by TLR3 induces receptor dimerization which results in inducing NF-κB activation (via TRIF-linked RIP1\/TLR3 interactions) and cytokine production. TLR3 has been shown to interact with a number of proteins including MYD88, TRAF6, TRIAD3, MAP3K7, and TAB2.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant mouse TLR3 extracellular domain.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): McCartney S, et al. 2009. J. Exp. Med. 13:2967. (IHC)\u003cbr\u003e\nProduct Citations: Chimote AA, et al. 2016. J Autoimmun. 86:69. PubMed Stone AEL, et al. 2019. Nat Commun. 10:3649. PubMed Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed\u003cbr\u003e\nRRID: AB_10895749 (BioLegend Cat. No. 141903) AB_10896420 (BioLegend Cat. No. 141904)\u003cbr\u003e\nStructure: Toll-like receptor family member; type I membrane protein containing multiple leucine repeats and a interleukin-1 receptor like region in the extracellular domain. Predicted molecular weight approximately 116 kD.\u003cbr\u003e\nDistribution: Selective expression in dendritic cells. Highly expressed in placenta and the pancreas; also expressed in heart, liver, lung and muscle.\u003cbr\u003e\nFunction: Pattern recognition receptor that participates in innate immune response to microbial pathogens. TLR3 ligands induce NF-κB (via TRIF-linked RIP1\/TLR3 interactions) and cytokine production. TLR3 stimulation results in the production of cytokines.\u003cbr\u003e\nInteraction: MYD88, TRAF6, TRIAD3, MAP3K7, and TAB2.\u003cbr\u003e\nLigand\/Receptor: Polyinosine-polycytidylic acid (Poly(I:C)), dsRNA.\u003cbr\u003e\nCell Type: Dendritic cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction\u003cbr\u003e\nMolecular Family: CD Molecules, Toll Like Receptors\u003cbr\u003e\nAntigen References: 1. Alexopoulou L, et al. 2001. Nature 413:732.2. Doyle SE, et al. 2002. Immunity 17:251.3. Meylan E, et al. 2004. Nat Immunol. 5:503.4. Muzio M, et al. 2000. J. Immunol. 164:5998.5. Rock FL, et al. 1998. P. Natl. Acad. Sci. USA 95:588.\u003cbr\u003e\nGene ID: 142980\u003cbr\u003e\nUniProt: View information about CD283 on UniProt.org\u003cbr\u003e\nClone: 11F8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Toll like receptor 3\u003cbr\u003e\nIsotype: Rat IgG2a\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860793708713,"sku":"141904","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-141904","provider":"Iright","version":"1.0","type":"link"}