{"product_id":"biolegend-142101","title":"Biolegend, 142101, Purified anti-mouse TIGIT (Vstm3) Antibody, 50μg","description":"\u003cp\u003eT cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as V-set and transmembrane domain-containing protein 3 (Vstm3), is a 26 kD, type I transmembrane protein and member of the CD28 family. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. Its binding partners include CD155 (PVR) and CD112 (PVRL2). TIGIT is a negative regulator of NK and T cell activation. Engagement of TIGIT by dendritic cells results in their differentiation into a tolerogenic phenotype, with an increased secretion of IL-10 and a diminished production of IL-12. Mice deficient for TIGIT are more susceptible to autoimmune disease.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Joller N, et al. 2010. J. Immunol. 186:1338.\u003cbr\u003e\nProduct Citations: Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed Shaffer TM, et al. 2021. Clin Cancer Res. 27:1932. PubMed Muhammad F, et al. 2020. J Autoimmun. 111:102441. PubMed\u003cbr\u003e\nRRID: AB_10983067 (BioLegend Cat. No. 142101) AB_10960124 (BioLegend Cat. No. 142102)\u003cbr\u003e\nStructure: Type I transmembrane protein, 26 kD, member of the CD28 family. Contains 1 Ig-like domain and 1 cytoplasmic ITIM motif.\u003cbr\u003e\nDistribution: Activated T cells, follicular T helper cells, NK, memory T cells, Tregs.\u003cbr\u003e\nFunction: Negative regulator of T cell activation and proliferation. Induction of tolerogenic dendritic cells.\u003cbr\u003e\nLigand\/Receptor: CD155 (PVR) and CD112 (PVRL2).\u003cbr\u003e\nCell Type: Dendritic cells, NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: Adhesion Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Levin SD, et al. 2011. Eur. J. Immunol. 41:902. 2. Yu X, et al. 2009. Nat. Immunol. 10:48. 3. Stanietsky N, et al. 2009. P. Natl. Acad. Sci. USA 106:17858.\u003cbr\u003e\nGene ID: 100043314\u003cbr\u003e\nUniProt: View information about TIGIT on UniProt.org\u003cbr\u003e\nClone: 1G9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: VSIG9\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860860424361,"sku":"142101","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-142101","provider":"Iright","version":"1.0","type":"link"}