{"product_id":"biolegend-142214","title":"Biolegend, 142214, PerCP\/Cyanine5.5 anti-mouse CD200R3 Antibody, 100μg","description":"\u003cp\u003eCD200R3, also known as CD200RLb and OX-2 Receptor 3, is a disulfide-linked dimeric CD200R-like receptor which belongs to immunoglobulin superfamily.  Its positively charged amino acid lysine associates with ITAM- or YxxM motif-bearing adaptor molecules such as DAP12, DAP10, FcRγ, and CD3ζ. CD200R3 functions as an activating receptor to regulate IgE independent immune response.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse primary basophils\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Ba13 recognizes circulating and bone marrow basophils; it also recognizes a subset of mast cells in the peritoneal cavity and skin. Additional reported applications (for the relevant formats) include: stimulation of bone marrow derived basophils to produce IL-4.\u003cbr\u003e\nProduct Citations: Xiao X, et al. 2024. J Cell Physiol. e31181:239. PubMed\u003cbr\u003e\nRRID: AB_2810409 (BioLegend Cat. No. 142213) AB_2810410 (BioLegend Cat. No. 142214)\u003cbr\u003e\nStructure: Immunoglobulin superfamily; disulfide-linked dimer with positively charge amino acids in transmembrane; molecular weight is about ~38 kD or ~74 kD (reduced or non-reduced conditions, respectively)\u003cbr\u003e\nDistribution: Basophils in peripheral blood, bone marrow, spleen; mast cells in peritoneal cavity and skin\u003cbr\u003e\nFunction: Transduce stimulatory signals\u003cbr\u003e\nInteraction: DAP12, DAP10, FcRγ, or CD3ζ\u003cbr\u003e\nCell Type: Basophils, Mast cells\u003cbr\u003e\nBiology Area: Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Voehringer D, et al. 2004. J. Biol. Chem. 52:54117. 2. Kojima T, et al. 2007. J. Immunol. 179:7093. 3. Sato K, et al. 2009. Blood 113:4780.\u003cbr\u003e\nGene ID: 74603\u003cbr\u003e\nUniProt: View information about CD200R3 on UniProt.org\u003cbr\u003e\nClone: Ba13\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD200 R3, CD200RLb, OX-2 Receptor 3, CD200 receptor 3\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862897774761,"sku":"142214","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-142214","provider":"Iright","version":"1.0","type":"link"}