{"product_id":"biolegend-142703","title":"Biolegend, 142703, PE anti-mouse TCR Vγ1.1 + Vγ1.2 Antibody, 25μg","description":"\u003cp\u003eIn mice, the TCR γ locus consists of seven Vγ, four Jγ, and four Cγ genes, which are differentially expressed throughout their lifespan. During fetal and early postnatal life, very few γ\/δ T cells express TCR Vγ1.1 or Vγ1.2 chains. In adult mice, however, a major fraction of the γ\/δ T cell repertoire comprises either TCR Vγ1.1 or Vγ1.2 chains. The Vγ1.1 gene rearranges almost exclusively with the Jγ4-Cγ4 genes, while the Vγ1.2 gene preferentially rearranges with Jγ1.  The 4B2.9 antibody reacts specifically with mouse TCR Vγ1.1 and Vγ1.2 as designated by the Garman nomenclature system. Mouse TCR Vγ1.1 and Vγ1.2 are also known as TCR Vγ1 and Vγ2, respectively, under the Heilig \u0026amp; Tonegawa nomenclature system.  Several TCR γ and δ chain nomenclature systems exist. In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts . \u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: TCR Vγ1.1\/Vδ6 T3.13.1 hybridoma\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: mAb 4B2.9 specifically recognizes γ\/δ T cells expressing Vγ1.1 and\/or Vγ1.2 chains.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Pereira P and Boucontet L. 2004. J. Immunol. 173:3261. (FC)\u003cbr\u003e\nProduct Citations: Giampaolo S, et al. 2023. iScience. 26:106234. PubMed Bachy E, et al. 2016. J Exp Med. 213: 841 - 857. PubMed\u003cbr\u003e\nRRID: AB_10960739 (BioLegend Cat. No. 142703) AB_10962688 (BioLegend Cat. No. 142704)\u003cbr\u003e\nDistribution: Subset of γ\/δ T cells\u003cbr\u003e\nFunction: Antigen recognition\u003cbr\u003e\nCell Type: T cells\u003cbr\u003e\nBiology Area: Adaptive Immunity, Immunology\u003cbr\u003e\nMolecular Family: TCRs\u003cbr\u003e\nAntigen References: 1. Garcia-Herrera A, et al. 2011. Am. J. Surg. Pathol. 35:1214. 2. Gutierrez A, et al. 2010. J. Clin. Oncol. 28:3816. 3. Zhang X, et al. 2009. Hematology 14:237.\u003cbr\u003e\nGene ID: 110067\u003cbr\u003e\nUniProt: View information about TCR Vgamma1.1 1.2 on UniProt.org\u003cbr\u003e\nClone: 4B2.9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Vγ1 and Vγ2 (Tonegawa nomenclature), TRGV1, TRGV2, GV5S1, GV5S2\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862410940585,"sku":"142703","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-142703","provider":"Iright","version":"1.0","type":"link"}