{"product_id":"biolegend-143506","title":"Biolegend, 143506, APC anti-mouse CD370 (CLEC9A, DNGR1) Antibody, 100μg","description":"\u003cp\u003eCD370 (CLEC9A), also known as DNGR1, is a 264 amino acid (aa) type II transmembrane glycoprotein which belongs to the C-type lectin superfamily. It contains a 208 aa extracellular region with a single carbohydrate recognition domain (CRD) and a cytoplasmic segment (35 aa) bearing an immunoreceptor tyrosine-based activation motif (ITAM).  In mice, CLEC9A is strictly expressed on CD8a-positive dendritic cells and plasmacytoid dendritic cells. It recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: ELISA2, immunofluorescence microcopy1, immunoprecipitation1, and Western blotting1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Sancho D, et al. 2008. J. Clin. Invest. 118:2098. (IF, IP, WB) Joffre OP, et al. 2010. Eur. J. Immunol. 40:1255. (ELISA)\u003cbr\u003e\nProduct Citations: Macri C, et al. 2024. NPJ Vaccines. 76:9. PubMed\u003cbr\u003e\nRRID: AB_2566379 (BioLegend Cat. No. 143505) AB_2566380 (BioLegend Cat. No. 143506)\u003cbr\u003e\nStructure: Type II transmembrane glycoprotein, consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD)\u003cbr\u003e\nDistribution: Strictly expressed on CD8α+ dendritic cells, migratory CD103+ dendritic cells, and plasmacytoid dendritic cells\u003cbr\u003e\nFunction: Cross present antigen to CTL\u003cbr\u003e\nInteraction: Necrotic cells\u003cbr\u003e\nLigand\/Receptor: Ubiquitous preformed acid-labile protein associated ligand(s)\u003cbr\u003e\nCell Type: Dendritic cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, MHC Antigens\u003cbr\u003e\nAntigen References: Poulin LF, et al. 2012. Blood 119:6052.\u003cbr\u003e\nGene ID: 232414\u003cbr\u003e\nUniProt: View information about CD370 on UniProt.org\u003cbr\u003e\nClone: 7H11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: DNGR-1, C-type lectin domain family 9 member A, CD370\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862489944233,"sku":"143506","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-143506","provider":"Iright","version":"1.0","type":"link"}